NPLOC4 Polyklonaler Antikörper

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• KD/KO Validated

NPLOC4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 11638-1-AP

Synonyme

KIAA1499, NPLOC4, Protein NPL4

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 11638-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11638-1-AP (NPLOC4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11638-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11638-1-AP (NPLOC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11638-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11638-1-AP (NPLOC4 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IP experiment of mouse kidney using 11638-1-AP

  IP Result of anti-NPLOC4 (IP:11638-1-AP, 4ug; Detection:11638-1-AP 1:600) with mouse kidney tissue lysate 6000ug.

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  IHC staining of human kidney using 11638-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11638-1-AP (NPLOC4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11638-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11638-1-AP (NPLOC4 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11638-1-AP bindet in WB, IP, IHC, IF, ELISA NPLOC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPLOC4 fusion protein Ag2219
• Vollständiger Name: nuclear protein localization 4 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 608 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC025930
• Gene symbol: NPLOC4
• Gene ID (NCBI): 55666
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IP	Science; Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.; Authors - Youngdae Gwon
mouse	WB	Sci Signal; TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing; Authors - Liangbin Lin
human	WB	J Biol Chem; Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.; Authors - Tao Wang
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KD Validated
human	WB	Mol Cell Biol; The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.; Authors - Rakesh Ganji ; KD Validated
human	WB	Virus Res; Involvement of VCP/UFD1/Nucleolin in the viral entry of Enterovirus A species.; Authors - Jingjing Yan ; KD Validated