NR1D1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NR1D1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(36)

Rezensionen (2)

Kat-Nr. : 14506-1-AP

Synonyme

ear 1, EAR1, EAR-1, HREV, Rev erbA alpha

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  WB analysis using 14506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 cells using 14506-1-AP

  WB result of anti-NR1D1 (14506-1-AP,1:1000) with A549 cell by Dr. Toryn Poolman.

• 

  WB analysis of A549 cells using 14506-1-AP

  WB result of anti-NR1D1 (14506-1-AP,1:500) with A549 treated with Cycloheximide by Dr. Toryn Poolman.

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  IHC staining of mouse skeletal muscle using 14506-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14506-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 14506-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 14506-1-AP (NR1D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 29 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• ChIP: See 5 publications below

Produktinformation

14506-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA NR1D1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR1D1 fusion protein Ag5964
• Vollständiger Name: nuclear receptor subfamily 1, group D, member 1
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC056148
• Gene symbol: NR1D1
• Gene ID (NCBI): 9572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.

Publikationen

Species	Application	Title
human	WB	Nat Commun; The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.; Authors - Yi Liu
mouse	WB	Theranostics; PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1.; Authors - Shuai Wang
human	WB,IF	Cancer Res; NR1D1 recruitment to sites of DNA damage inhibits repair and is associated with chemosensitivity of breast cancer.; Authors - Na-Lee Ka
human	WB	Int J Biol Macromol; Dihydroartemisinin requires NR1D1 mediated Rab7 ubiquitination to regulate hepatic stellate cells lipophagy in liver fibrosis; Authors - Zhengyang Bao ; KD Validated
mouse	WB	Cell Death Discov; NR1D1 deficiency in the tumor microenvironment promotes lung tumor development by activating the NLRP3 inflammasome; Authors - Sun Mi Kim
mouse	WB	J Neuroinflammation; The circadian clock protein Rev-erbα provides neuroprotection and attenuates neuroinflammation against Parkinson's disease via the microglial NLRP3 inflammasome.; Authors - Liang Kou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (01-17-2023)

I have used for cardiomyocytes, mouse skin and liver tissues

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, mouse Liver and skin

FH

Satish (Verified Customer) (12-19-2019)

I got clear bands. The antibody detects two band one band around 67 kDa and another band much lower than 67kDa.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: INS1 cells