GCNF Polyklonaler Antikörper

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• KD/KO Validated

GCNF Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12712-1-AP

Synonyme

GCNF, GCNF1, Germ cell nuclear factor, hGCNF, hRTR, NR61, NR6A1, RTR

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 12712-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12712-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12712-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12712-1-AP

  IP Result of anti-GCNF (IP:12712-1-AP, 4ug; Detection:12712-1-AP 1:800) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 12712-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12712-1-AP (GCNF antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 12712-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12712-1-AP (GCNF antibody at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, K-562-Zellen, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12712-1-AP bindet in WB, IP, IHC, IF, ELISA GCNF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GCNF fusion protein Ag3395
• Vollständiger Name: nuclear receptor subfamily 6, group A, member 1
• Berechnetes Molekulargewicht: 475 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC030600
• Gene symbol: NR6A1
• Gene ID (NCBI): 2649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR6A1 (Nuclear Receptor Subfamily 6 Group A member), also known as GCNF (Germ Cell Nuclear Factor), is an orphan member of the nuclear receptor gene superfamily. It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation. Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death. GCNF has been shown to be a repressor of OCT-4 and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis. This antibody specifically reacts with the 55kd NR6A1 protein.

Publikationen

Species	Application	Title
mouse	WB,IF	Neuropharmacology; Hippocampal NR6A1 impairs CREB-BDNF signaling and leads to the development of depression-like behaviors in mice.; Authors - Pingping Tan ; KD Validated
mouse	IHC	Cancer Med; microRNA-196a-5p inhibits testicular germ cell tumor progression via NR6A1/E-cadherin axis.; Authors - Xiaowen Liu ; KD Validated
human	WB	J Clin Transl Hepatol; Establishment of a Prognostic Model for Hepatocellular Carcinoma Based on Bioinformatics and the Role of NR6A1 in the Progression of HCC; Authors - Zhong-Hua Lin