NRCAM Polyklonaler Antikörper

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• KD/KO Validated

NRCAM Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 21608-1-AP

Synonyme

hBravo, KIAA0343, neuronal cell adhesion molecule, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

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Galerie der Validierungsdaten

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  WB analysis using 21608-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21608-1-AP (NRCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21608-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21608-1-AP (NRCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21608-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21608-1-AP (NRCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21608-1-AP (NRCAM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21608-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21608-1-AP (NRCAM antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 21608-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 21608-1-AP (NRCAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

21608-1-AP bindet in WB, IHC, IF, ELISA NRCAM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NRCAM fusion protein Ag16237
• Vollständiger Name: neuronal cell adhesion molecule
• Berechnetes Molekulargewicht: 1304 aa, 144 kDa
• Beobachtetes Molekulargewicht: 130-136 kDa
• GenBank-Zugangsnummer: BC098401
• Gene symbol: NRCAM
• Gene ID (NCBI): 4897
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).

Publikationen

Species	Application	Title
human	IF	Stem Cell Reports; CD73 Regulates Stemness and Epithelial-Mesenchymal Transition in Ovarian Cancer-Initiating Cells.; Authors - Michela Lupia
human	WB	Mol Cell Proteomics; Application of mass spectrometry profiling to establish brusatol as an inhibitor of global protein synthesis.; Authors - Steffan Vartanian
human	WB	Front Oncol; An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.; Authors - Hongwei Jin
human	WB	Cancer Gene Ther; NrCAM secreted by endometrial stromal cells enhances the progestin sensitivity of endometrial cancer cells through epigenetic modulation of PRB.; Authors - Yali Cheng ; KD Validated
mouse	IHC	Mol Cell Neurosci; Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.; Authors - Zhen-Huang Chen