NRF1/nuclear respiratory factor 1 Polyklonaler Antikörper

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• KD/KO Validated

NRF1/nuclear respiratory factor 1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(99)

Kat-Nr. : 12482-1-AP

Synonyme

NRF1, nuclear respiratory factor 1, ALPHA PAL, Alpha palindromic-binding protein, Alpha-pal

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  WB analysis using 12482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12482-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 12482-1-AP

  IP result of anti-NRF1/nuclear respiratory factor 1 (IP:12482-1-AP, 4ug; Detection:12482-1-AP 1:300) with mouse skeletal muscle tissue lysate 3000ug.

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  IHC staining of human stomach using 12482-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293T-Zellen, humanes Lungengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 96 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12482-1-AP bindet in WB, IHC, IF, ELISA NRF1/nuclear respiratory factor 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Hausschwein, Huhn, Maus, Ratte, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NRF1/nuclear respiratory factor 1 fusion protein Ag3172
• Vollständiger Name: nuclear respiratory factor 1
• Berechnetes Molekulargewicht: 522 aa, 56 kDa
• Beobachtetes Molekulargewicht: 67 kDa, 45 kDa
• GenBank-Zugangsnummer: BC016925
• Gene symbol: NRF1
• Gene ID (NCBI): 4899
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Publikationen

Species	Application	Title
mouse	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
chicken	WB,IHC	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong
	WB	J Autoimmun; Autoantibodies against the NineTeen complex and U5 RNP in systemic sclerosis; Authors - Jean-Baptiste Vulsteke
rat,human	WB	J Adv Res; A dual absorption pathway of novel oyster-derived peptide-zinc complex enhances zinc bioavailability and restores mitochondrial function; Authors - Ximing Yang
human	WB	Redox Biol; Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy.; Authors - Hao Wu
chicken	WB	Cell Commun Signal; Local GHR roles in regulation of mitochondrial function through mitochondrial biogenesis during myoblast differentiation; Authors - Bowen Hu