NUB1 Polyklonaler Antikörper

NUB1 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14343-1-AP

Synonyme

BS4, NEDD8 ultimate buster 1, NUB1, NUB1L, NYREN18

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14343-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14343-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14343-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14343-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14343-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14343-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14343-1-AP

  IP Result of anti-NUB1 (IP:14343-1-AP, 3ug; Detection:14343-1-AP 1:600) with HEK-293 cells lysate 2500ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HepG2-Zellen, K-562-Zellen, Mausnierengewebe, Raji-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14343-1-AP bindet in WB, IP, IHC, IF, ELISA NUB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUB1 fusion protein Ag5668
• Vollständiger Name: negative regulator of ubiquitin-like proteins 1
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC046354
• Gene symbol: NUB1
• Gene ID (NCBI): 51667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proteasome-associated protein NEDD8 ultimate buster 1 (NUB1) is an interferon-inducible protein that down-regulates NEDD8 expression and its conjugation system. Over-expression of NUB1 induces a growth-inhibitory effect in cells, and it mediates anti-proliferative actions and apoptosis in renal cell carcinoma cells through cell-cycle regulation. Expression of NUB1 leads to decreased modification of p53 with NEDD8 and stimulation of p53 ubiquitination. Recent finding revealed that NUB1, by regulating GSK3β levels, modulates tau phosphorylation and aggregation, and is a key player in neurodegeneration associated with tau pathology.

Publikationen

Species	Application	Title
mouse	WB,IF	Biochem Biophys Res Commun; The role of NUB1 in α-synuclein degradation in Lewy body disease model mice.; Authors - Kunikazu Tanji
human	IHC	Neuropathology; Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.; Authors - Fumiaki Mori
human	WB	Brain Pathol; Phosphorylated NUB1 distinguishes α-synuclein in Lewy bodies from that in glial cytoplasmic inclusions in multiple system atrophy; Authors - Kunikazu Tanji

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