NUCKS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NUCKS1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12023-2-AP

Synonyme

JC7, NUCKS, NUCKS1, P1

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Galerie der Validierungsdaten

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  WB analysis using 12023-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12023-2-AP (NUCKS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12023-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12023-2-AP (NUCKS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12023-2-AP

  IP result of anti-NUCKS1(IP:12023-2-AP, 4ug; Detection:12023-2-AP 1:600) with HEK-293 cells lysate 960 ug.

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  IHC staining of human gliomas using 12023-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat pancreas using 12023-2-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat pancreas using 12023-2-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12023-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HEK-293-Zellen, MCF-7-Zellen, SW 1990-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Ratten-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

12023-2-AP bindet in WB, IP, IHC, IF, ChIP, ELISA NUCKS1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUCKS1 fusion protein Ag2652
• Vollständiger Name: nuclear casein kinase and cyclin-dependent kinase substrate 1
• Berechnetes Molekulargewicht: 243 aa, 27 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC000805
• Gene symbol: NUCKS1
• Gene ID (NCBI): 64710
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (NUCKS1) is a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated by CDK1 and casein kinase during mitosis of the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Widelyexpressed, with highest levels in thyroid gland, prostate and uterus and in fetal liver, thymus and lung. Two isoforms of NUCKS1 exist due to alternative splicing events.

Publikationen

Species	Application	Title
human	ChIP	Nat Commun; The NUCKS1-SKP2-p21/p27 axis controls S phase entry.; Authors - Samuel Hume
human	WB	J Exp Clin Cancer Res; NUCKS promotes cell proliferation and suppresses autophagy through the mTOR-Beclin1 pathway in gastric cancer.; Authors - Erhu Zhao ; KD Validated
bovine	WB,IF	J Cell Physiol; NUCKS1 is a novel regulator of milk synthesis in and proliferation of mammary epithelial cells via the mTOR signaling pathway.; Authors - Xiaohan Yuan
human,mouse	WB,ChIP,IHC	Cell Death Dis; NUCKS1, a LINC00629-upregulated gene, facilitated osteosarcoma progression and metastasis by elevating asparagine synthesis; Authors - Shuo Zheng ; KD Validated