NUDC Polyklonaler Antikörper

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• KD/KO Validated

NUDC Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10681-1-AP

Synonyme

HNUDC, MNUDC, Nuclear migration protein nudC, NUDC

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 10681-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10681-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10681-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10681-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10681-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10681-1-AP (NUDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10681-1-AP

  IP Result of anti-NUDC (IP:10681-1-AP, 4ug; Detection:10681-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human pancreas cancer using 10681-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10681-1-AP (NUDC antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 10681-1-AP

  Immunofluorescent analysis of HeLa cells using 10681-1-AP (NUDC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10681-1-AP

  Immunofluorescent analysis of HepG2 cells using 10681-1-AP (NUDC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10681-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10681-1-AP (NUDC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

10681-1-AP bindet in WB, IP, IHC, IF,ELISA NUDC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUDC fusion protein Ag0998
• Vollständiger Name: nuclear distribution gene C homolog (A. nidulans)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 42-45 kDa
• GenBank-Zugangsnummer: BC006147
• Gene symbol: NUDC
• Gene ID (NCBI): 10726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear distribution protein C (NudC) is a highly conserved dynein/dynactin associated protein implicated in mitosis and cytokinesis. NudC is localized on major mitotic structures, and is involved in regulating spindle formation stability of kinetochore microtubule attachments, and chromosomes congression in early mitosis. NudC also is required for multi-protein transport along axons in neurons.

Publikationen

Species	Application	Title
human	WB,IF	Cell Res; NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.; Authors - Cheng Zhang ; KD Validated
human	IF	Int J Mol Sci; N-Acetyl-D-Glucosamine Kinase Interacts with NudC and Lis1 in Dynein Motor Complex and Promotes Cell Migration.; Authors - Md Ariful Islam
human	WB	Mol Cell Biol; Substrate Trapping Proteomics Reveals Targets of the βTrCP2/FBXW11 Ubiquitin Ligase.; Authors - Tai Young Kim
human	WB	Front Cell Dev Biol; NudC L279P Mutation Destabilizes Filamin A by Inhibiting the Hsp90 Chaperoning Pathway and Suppresses Cell Migration.; Authors - Min Liu
mouse	WB,IHC	Reprod Domest Anim; Proteomic-based identification of oocyte maturation-related proteins in mouse germinal vesicle oocytes.; Authors - Senyang Cao