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  • KD/KO Validated

NUF2 Polyklonaler Antikörper

NUF2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 15731-1-AP

Synonyme

CDCA1, hNuf2, hNuf2R, hsNuf2, Kinetochore protein Nuf2, NUF2, NUF2R



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Lebergewebe, humanes Skelettmuskelgewebe, humanes Hodengewebe, PC-3-Zellen
Erfolgreiche IPHEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15731-1-AP bindet in WB, IHC, IP, ELISA NUF2 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NUF2 fusion protein Ag8479
Vollständiger Name NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Berechnetes Molekulargewicht 464 aa, 54 kDa
Beobachtetes Molekulargewicht 50 kDa
GenBank-ZugangsnummerBC008489
Gene symbol NUF2
Gene ID (NCBI) 83540
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. NUF2 was reported to be overexpressed in various kinds of carcinomas including lung cancer, cholangiocellular cancer, urinary bladder cancer, renal cell cancer, colorectal cancer, gastric cancer, ovarian cancer and serous adenocarcinoma. Elevated expression of NUF2 was associated with poorer prognosis of non-small cell lung cancer patients (PMID: 17079454). Knockdown of NUF2 significantly caused cell growth delay and increased apoptosis in cell lines of non-small cell lung cancer, colorectal cancer, gastric cancer and ovarian cancer.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for NUF2 antibody 15731-1-APProtokoll herunterladen
IP protocol for NUF2 antibody 15731-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Death Dis

LncRNA CDKN2B-AS1 stabilized by IGF2BP3 drives the malignancy of renal clear cell carcinoma through epigenetically activating NUF2 transcription.

Authors - Xina Xie
humanWB,IHC

Int J Biol Sci

NUF2 Drives Clear Cell Renal Cell Carcinoma by Activating HMGA2 Transcription through KDM2A-mediated H3K36me2 Demethylation

Authors - Jiatian Lin
  • KD Validated
human,mouseWB

Front Oncol

NUF2 overexpression contributes to epithelial ovarian cancer progression via ERBB3-mediated PI3K-AKT and MAPK signaling axes

Authors - Ruobing Leng
  • KD Validated
humanWB

Adv Sci (Weinh)

CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC

Authors - Qi Li
humanWB,IHC

Carcinogenesis

NUF2 and NEK2 promote malignant progression of gallbladder cancer by remodeling the extracellular matrix

Authors - Ming Gao
humanWB,IP,IHC

Biochem Pharmacol

NUF2 activated by YY1 promotes prostate cancer malignancy via p38/MAPK signaling axis and serves as a therapeutic target

Authors - Chengqi Jin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Katie (Verified Customer) (05-23-2022)

Works well for western

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T cells