SLC13A3 Polyklonaler Antikörper

SLC13A3 Polyklonal Antikörper für IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 26184-1-AP

Synonyme

hNaDC3, NaDC 3, NADC3, SDCT2, SLC13A3

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  IP experiment of mouse kidney using 26184-1-AP

  IP result of anti-SLC13A3 (IP:26184-1-AP, 4ug; Detection:26184-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 26184-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (NaDC3 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26184-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (NaDC3 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 26184-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (SLC13A3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26184-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26184-1-AP (SLC13A3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

26184-1-AP bindet in WB, IHC, IF, IP, ELISA SLC13A3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC13A3 fusion protein Ag24182
• Vollständiger Name: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3
• Berechnetes Molekulargewicht: 602 aa, 67 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC035966
• Gene symbol: SLC13A3
• Gene ID (NCBI): 64849
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC13A3, also named NADC3 and SDCT2, belongs to the SLC13A/DASS transporter (TC 2.A.47) family and NADC subfamily. SLC13A3 encodes the plasma membrane Na+ /Dicarboxylate Cotransporter 3 (NaDC3), which imports inside the cell four to six carbon dicarboxylates as well as N-acetylaspartate (NAA). SLC13A3 is mainly expressed in kidney, but also in brain, liver, placenta and eye (PMID: 30635937).

Publikationen

Species	Application	Title
rat	WB	Bioact Mater; An Injectable silk-based hydrogel as a novel biomineralization seedbed for critical-sized bone defect regeneration; Authors - Yuhui Zhu
human	WB,IF	Dev Cell; Itaconate uptake via SLC13A3 improves hepatic antibacterial innate immunity; Authors - Chao Chen ; KO Validated
human	WB	Adv Sci (Weinh); Transcriptomic Profiling Unveils EDN3+ Meningeal Fibroblasts as Key Players in Sturge-Weber Syndrome Pathogenesis; Authors - Daosheng Ai
human,mouse	WB,IHC	Cell Metab; Itaconate transporter SLC13A3 confers immunotherapy resistance via alkylation-mediated stabilization of PD-L1; Authors - Yizeng Fan