Neutrophil Elastase Rekombinanter Antikörper

Neutrophil Elastase Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

250346G5

Kat-Nr. : 98499-1-RR

Synonyme

ELA2, ELANE, EC:3.4.21.37, Elastase 2, HLE

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  FC experiment of human peripheral blood leukocytes using 98499-1-RR

  1x10^6 human peripheral blood leukocytes were intracellularly stained with 0.25 ug Anti-Human Neutrophil Elastase Rabbit RecAb (98499-1-RR, Clone: 250346G5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of U-937 using 98499-1-RR

  1x10^6 U-937 cells (left) or Jurkat cells (right) were intracellularly stained with 0.25 ug Anti-Human Neutrophil Elastase Rabbit RecAb (98499-1-RR, Clone: 250346G5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): human peripheral blood leukocytes, U-937-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98499-1-RR bindet in FC (Intra) Neutrophil Elastase und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Neutrophil Elastase fusion protein Eg3192
• Vollständiger Name: elastase 2, neutrophil
• Berechnetes Molekulargewicht: 29KD
• GenBank-Zugangsnummer: NM_001972.4
• Gene symbol: ELA2
• Gene ID (NCBI): 1991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.