Neutrophil elastase Rekombinanter Antikörper

Neutrophil elastase Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

241842G3

Kat-Nr. : FITC-98197

Synonyme

241842G3, EC:3.4.21.37, Ela2, Elane, Elastase-2

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  FC experiment of mouse bone marrow cells using FITC-98197

  1x10^6 mouse bone marrow cells (left) or NIH/3T3 cells (right) were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse Neutrophil elastase Rabbit RecAb (FITC-98197, Clone: 241842G3) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-98197 bindet in FC (Intra) Neutrophil elastase und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Neutrophil elastase fusion protein Eg2126
• Vollständiger Name: elastase 2, neutrophil
• Berechnetes Molekulargewicht: 29KD
• GenBank-Zugangsnummer: NM_015779.2
• Gene symbol: Ela2
• Gene ID (NCBI): 50701
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

ELA2 (Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation (PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation) (PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration (PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.