NUCB2/nesfatin-1 Polyklonaler Antikörper

NUCB2/nesfatin-1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 26712-1-AP

Synonyme

NUCB2, DNA binding protein NEFA, Gastric cancer antigen Zg4, NEFA, nucleobindin 2

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  WB analysis using 26712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26712-1-AP

  Human milk were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26712-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 26712-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 26712-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 26712-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26712-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUCB2/nesfatin-1 antibody (26712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 26712-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293T-Zellen, HepG2-Zellen, Mausmilzgewebe, Maushodengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

26712-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA NUCB2/nesfatin-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUCB2/nesfatin-1 fusion protein Ag25152
• Vollständiger Name: nucleobindin 2
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: NM_005013
• Gene symbol: NUCB2
• Gene ID (NCBI): 4925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

Publikationen

Species	Application	Title
human	WB	Proteomics; PMID: 29901268; Authors - Wang Li
human	WB	J Transl Med; NUCB2/Nesfatin-1 drives breast cancer metastasis through the up-regulation of cholesterol synthesis via the mTORC1 pathway; Authors - Siyi Ning
rat,mouse	WB,IF	Cell Commun Signal; Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling; Authors - Xue-Xue Zhu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

Clear and specific band

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

NUCB2/nesfatin-1 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:26712-1-AP)