NUCB2/nesfatin-1 Polyklonaler Antikörper

NUCB2/nesfatin-1 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 26712-1-AP

Synonyme

DNA binding protein NEFA, Gastric cancer antigen Zg4, NEFA, NUCB2, nucleobindin 2

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Galerie der Validierungsdaten

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  WB analysis using 26712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 26712-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26712-1-AP

  Human milk were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 26712-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 26712-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 26712-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 26712-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293T-Zellen, HepG2-Zellen, Maushodengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

26712-1-AP bindet in WB, IF, ELISA NUCB2/nesfatin-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUCB2/nesfatin-1 fusion protein Ag25152
• Vollständiger Name: nucleobindin 2
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: NM_005013
• Gene symbol: NUCB2
• Gene ID (NCBI): 4925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

Publikationen

Species	Application	Title
human	WB	Proteomics; PMID: 29901268; Authors - Wang Li
human	WB	J Transl Med; NUCB2/Nesfatin-1 drives breast cancer metastasis through the up-regulation of cholesterol synthesis via the mTORC1 pathway; Authors - Siyi Ning

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

Clear and specific band

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

NUCB2/nesfatin-1 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:26712-1-AP)