Occludin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Occludin Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

367

Rezensionen (5)

Kat-Nr. : 13409-1-AP

Synonyme

occludin, OCLN

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Galerie der Validierungsdaten

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  WB analysis using 13409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 13409-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13409-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13409-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13409-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13409-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13409-1-AP

  IP Result of anti-Occludin (IP:13409-1-AP, 3ug; Detection:13409-1-AP 1:500) with mouse liver tissue lysate 6400ug.

• 

  IHC staining of human breast cancer using 13409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 13409-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (13409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 13409-1-AP

  1X10^6 A431 cells were stained with 0.2 ug Anti-Human Occludin (13409-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, humanes Lebergewebe, humanes Nierengewebe, Maus-Kolongewebe, Mauslebergewebe, Mausnierengewebe, Rattenlebergewebe, Rattennierengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:800
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 298 publications below
• IHC: See 49 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

13409-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Occludin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Hund, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Occludin fusion protein Ag4057
• Vollständiger Name: occludin
• Berechnetes Molekulargewicht: 522 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC029886
• Gene symbol: OCLN
• Gene ID (NCBI): 4950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Publikationen

Species	Application	Title
mouse	IHC	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
human	WB	Hepatology; Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.; Authors - Meng Lu
mouse	WB	J Exp Med; Brain endothelial TAK1 and NEMO safeguard the neurovascular unit.; Authors - Dirk A Ridder
human	WB	Nat Commun; Daxx inhibits hypoxia-induced lung cancer cell metastasis by suppressing the HIF-1α/HDAC1/Slug axis.; Authors - Ching-Wen Lin
human	WB	Mater Today Bio; ROS-responsive nanoparticles for oral delivery of luteolin and targeted therapy of ulcerative colitis by regulating pathological microenvironment.; Authors - Chen Tan
mouse		EMBO Mol Med; Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.; Authors - Ming Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (02-03-2023)

1:5000 on murine intestinal protein extract for 2h in TBST

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse intestines

FH

Wu (Verified Customer) (12-09-2021)

The antibody gives a good detection of Occludin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: gut

Occludin Antibody Immunofluorescence validation (1:100 dilution) in gut (Cat no:13409-1-AP)

FH

Tom (Verified Customer) (09-21-2021)

Gave decent results with IHC at a concentration of 1:300-1:400 for mouse intestinal tissue

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:300-1:400
• Cell Tissue Type: Mouse intestinal tissue

FH

Amy (Verified Customer) (05-22-2019)

I approached Proteintech for guidance with regards to dilutions of this antibody, and with their help I optimized the protocol in good time. Very helpful!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse Colon

FH

JIMMY (Verified Customer) (03-26-2019)

This antibody offers a good detection of Occludin on mouse brain endothelial cell line, bEnd3 cells, through IHC.

• Applications: Immunofluorescence,

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