OMA1 Polyklonaler Antikörper

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• KD/KO Validated

OMA1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 17116-1-AP

Synonyme

2010001O09Rik, DAB1, MPRP 1, MPRP1, OMA1, YKR087C, ZMPOMA1

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Galerie der Validierungsdaten

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  WB analysis using 17116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17116-1-AP (OMA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17116-1-AP (OMA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17116-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17116-1-AP (OMA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17116-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17116-1-AP (OMA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17116-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17116-1-AP (OMA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, HepG2-Zellen, K-562-Zellen, MDA-MB-231-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 19 publications below

Produktinformation

17116-1-AP bindet in WB, IHC, ELISA OMA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OMA1 fusion protein Ag10803
• Vollständiger Name: OMA1 homolog, zinc metallopeptidase (S. cerevisiae)
• Berechnetes Molekulargewicht: 524 aa, 60 kDa
• Beobachtetes Molekulargewicht: 34, 60 kDa
• GenBank-Zugangsnummer: BC012915
• Gene symbol: OMA1
• Gene ID (NCBI): 115209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OMA1 (Overlapping with the m-AAA protease 1 homolog) is also named as MPRP1 (Metalloprotease-related protein 1) and belongs to the peptidase M48 family. It is a zinc metalloprotease that spans the inner mitochondrial membrane with a number of predicted membrane spanning domains and the 60 kDa form of OMA1 rapidly accumulated concomitantly with the cleavage of all OPA1 variants, the 40 kDa form of OMA1 may be the mature form (M-OMA1), and the 35 kDa is a cleaved form (S-OMA1), with the 60 kDa form being the active enzyme (PMID:20334834, 30518622). This antibody is speicific to OMA1.

Publikationen

Species	Application	Title
human	WB	Nature; A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.; Authors - Evelyn Fessler
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
human	WB	Leukemia; DELE1 haploinsufficiency causes resistance to mitochondrial stress-induced apoptosis in monosomy 5/del(5q) AML; Authors - Jean-François Spinella
mouse	WB	Redox Biol; Peroxiredoxin 3 deficiency induces cardiac hypertrophy and dysfunction by impaired mitochondrial quality control.; Authors - Seong Keun Sonn ; KD Validated
human	WB	Cell Death Dis; Inhibition of the mitochondria-shaping protein Opa1 restores sensitivity to Gefitinib in a lung adenocarcinomaresistant cell line; Authors - Masafumi Noguchi
human	WB	Cell Rep; TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria; Authors - Shiori Akabane