ONECUT1 Polyklonaler Antikörper

ONECUT1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 25137-1-AP

Synonyme

Hepatocyte nuclear factor 6, HNF 6, HNF6, HNF6A, One cut domain family member 1, one cut homeobox 1, ONECUT1

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  WB analysis of mouse liver using 25137-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25137-1-AP (ONECUT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

25137-1-AP bindet in WB, IHC, IF, ELISA ONECUT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ONECUT1 fusion protein Ag18250
• Vollständiger Name: one cut homeobox 1
• Berechnetes Molekulargewicht: 465 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51-55 kDa
• GenBank-Zugangsnummer: BC140830
• Gene symbol: ONECUT1
• Gene ID (NCBI): 3175
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ONECUT1, also named as Hepatocyte nuclear factor 6 (HNF6), is a 465 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT1 localizes in the nucleus and belongs to the CUT homeobox family. ONECUT1 as a transcription activator is involved in the some liver genes transcription. ONECUT1 is highly expressed in liver and lower in testis, skin.

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; REVERBa couples the circadian clock to hepatic glucocorticoid action.; Authors - Giorgio Caratti
human	WB	J Virol; Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatocyte Nuclear Factor 6.; Authors - Ruidong Hao
human	WB	Virol Sin; Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters; Authors - Xinna Ma
mouse	IF	bioRxiv; Enhanced Plasmid-Based Transcriptional Activation in Developing Mouse Photoreceptors; Authors - Brendon M Patierno
zebrafish	WB	Cells; The Zebrafish Retina and the Evolution of the Onecut-Mediated Pathway in Cell Type Differentiation; Authors - Quirino Attilio Vassalli
human	IF	Invest Ophthalmol Vis Sci; Enhanced Transcriptional Activation in Developing Mouse Photoreceptors; Authors - Brendon M Patierno