ONECUT2 Polyklonaler Antikörper

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• KD/KO Validated

ONECUT2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Kat-Nr. : 21916-1-AP

Synonyme

OC2, OC 2, HNF-6-beta, HNF6B, HNF 6 beta

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  WB analysis using 21916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 21916-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21916-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21916-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 21916-1-AP

  IP result of anti-ONECUT2 (IP:21916-1-AP, 4ug; Detection:21916-1-AP 1:300) with HEK-293 cells lysate 3200ug.

• 

  IHC staining of human stomach cancer using 21916-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21916-1-AP (ONECUT2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushautgewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 6 publications below
• IP: See 1 publications below
• ChIP: See 4 publications below

Produktinformation

21916-1-AP bindet in WB, IHC, IP, chIP, ELISA ONECUT2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ONECUT2 fusion protein Ag16162
• Vollständiger Name: one cut homeobox 2
• Berechnetes Molekulargewicht: 504 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54-60 kDa
• GenBank-Zugangsnummer: BC108925
• Gene symbol: ONECUT2
• Gene ID (NCBI): 9480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ONECUT2, also named as One cut domain family member 2 or Hepatocyte nuclear factor 6-beta, is a 504 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT2 belongs to the CUT homeobox family and activates the transcription of a number of liver genes such as HNF3B. ONECUT2 is required for liver differnetiation and metabolism. ONECUT2 and ONECUT3 play redundant roles with ONECUT1 in pancreas development.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Temporal evolution reveals bifurcated lineages in aggressive neuroendocrine small cell prostate cancer trans-differentiation; Authors - Chia-Chun Chen
human	WB	Nat Med; ONECUT2 is a targetable master regulator of lethal prostate cancer that suppresses the androgen axis.; Authors - Mirja Rotinen
human	IHC	Cancer Cell; Epigenomic State Transitions Characterize Tumor Progression in Mouse Lung Adenocarcinoma.; Authors - Lindsay M LaFave
human,mouse	WB	Nat Commun; Neuronal miR-9 promotes HSV-1 epigenetic silencing and latency by repressing Oct-1 and Onecut family genes; Authors - Yue Deng
human	WB	Sci Adv; Hypoxia-mediated stabilization of HIF1A in prostatic intraepithelial neoplasia promotes cell plasticity and malignant progression.; Authors - Mohamed A Abu El Maaty
human	WB	Proc Natl Acad Sci U S A; Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.; Authors - Lei Shi ; KD Validated