OPTN/Optineurin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OPTN/Optineurin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(128)

Rezensionen (1)

Kat-Nr. : 10837-1-AP

Synonyme

Optineurin, OPTN, ALS12, E3-14.7K-interacting protein, FAA4

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  WB analysis using 10837-1-AP

  various lysates were subjected to SDS PAGE followed by western blot with 10837-1-AP (OPTN antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10837-1-AP

  WB result of OPTN antibody (10837-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPTN transfected HeLa cells.

• 

  WB analysis using 10837-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10837-1-AP (OPTN/Optineurin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10837-1-AP

  IP result of anti-OPTN/Optineurin (IP:10837-1-AP, 4ug; Detection:10837-1-AP 1:10000) with HeLa cells lysate 1400 ug.

• 

  IHC staining of human gliomas using 10837-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human gliomas using 10837-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of rat eye using 10837-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed paraffin-embedded rat eye tissue using OPTN antibody (10837-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10837-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OPTN antibody (10837-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HeLa using 10837-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OPTN (10837-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushirngewebe, NCCIT-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Ratten-Augengewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 15 publications below
• WB: See 105 publications below
• IHC: See 8 publications below
• IF: See 22 publications below
• IP: See 7 publications below
• CoIP: See 4 publications below

Produktinformation

10837-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA OPTN/Optineurin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, hamster, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OPTN/Optineurin fusion protein Ag1272
• Vollständiger Name: optineurin
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC013876
• Gene symbol: OPTN
• Gene ID (NCBI): 10133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.

Publikationen

Species	Application	Title
human	WB	Nature; The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy.; Authors - Michael Lazarou
mouse	WB	Cell; NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.; Authors - Zhenyu Zhong
human	CoIP	Mol Cell; TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1; Authors - François Le Guerroué
human	IF	Acta Neuropathol; p62 positive, TDP-43 negative, neuronal cytoplasmic and intranuclear inclusions in the cerebellum and hippocampus define the pathology of C9orf72-linked FTLD and MND/ALS.; Authors - Safa Al-Sarraj
Human	IHC	Acta Neuropathol; Optineurin inclusions occur in a minority of TDP-43 positive ALS and FTLD-TDP cases and are rarely observed in other neurodegenerative disorders.; Authors - Tibor Hortobágyi
human	WB	Mol Cell; Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain.; Authors - Shireen A Sarraf ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Muhammed Talha (Verified Customer) (08-31-2023)

It worked very well at 4 degree for overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: hela

OPTN/Optineurin Antibody Western Blot validation (1:5000 dilution) in hela (Cat no:10837-1-AP)