OPTN/Optineurin Polyklonaler Antikörper

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• KD/KO Validated

OPTN/Optineurin Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10837

Synonyme

Optineurin, OPTN, FIP-2, FIP2, FIP 2

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  IF Staining of MCF-7 using CL488-10837

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 OPTN antibody (CL488-10837) at dilution of 1:200.

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  FC experiment of HeLa using CL488-10837

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human OPTN (CL488-10837) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10837 bindet in IF/ICC, FC (Intra) OPTN/Optineurin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OPTN/Optineurin fusion protein Ag1272
• Vollständiger Name: optineurin
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa, 70-74 kDa
• GenBank-Zugangsnummer: BC013876
• Gene symbol: OPTN
• Gene ID (NCBI): 10133
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.