ORAI1 Monoklonaler Antikörper
ORAI1 Monoklonal Antikörper für WB, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
Hausschwein, human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
3C12F9
Kat-Nr. : 66223-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HL-60-Zellen, 4T1-Zellen, A375-Zellen, Daudi-Zellen, Jurkat-Zellen, K-562-Zellen, Hausschwein-Milzgewebe, Hausschwein-Thymusgewebe, Raji-Zellen, Ramos-Zellen, Rattenmilzgewebe, RAW 264.7-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:30000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 23 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Produktinformation
66223-1-Ig bindet in WB, IHC, IF, ELISA ORAI1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Affe, Maus, Ratte, Rind |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | ORAI1 fusion protein Ag18050 |
| Vollständiger Name | ORAI calcium release-activated calcium modulator 1 |
| Berechnetes Molekulargewicht | 301 aa, 33 kDa |
| Beobachtetes Molekulargewicht | 32 kDa |
| GenBank-Zugangsnummer | BC015369 |
| Gene symbol | ORAI1 |
| Gene ID (NCBI) | 84876 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for ORAI1 antibody 66223-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cell Death Dis Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats | ||
Acta Pharmacol Sin Endothelial cell Orai1 is essential for endothelium-dependent contraction of mouse carotid arteries in normotensive and hypertensive mice | ||
Oncogenesis Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth | ||
Int J Mol Sci Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y | ||
Front Cell Dev Biol Transmembrane Protein Ttyh1 Maintains the Quiescence of Neural Stem Cells Through Ca2+/NFATc3 Signaling. | ||
J Innate Immun Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Udesh (Verified Customer) (01-19-2023) | The Ab worked well for WB. 20ug protein loaded. Probed overnight at 4 deg.
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