ORAI1 Monoklonaler Antikörper

ORAI1 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (2)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

CloneNo.

3C12F9

Rezensionen (1)

Kat-Nr. : 66223-1-Ig

Synonyme

ORAI1

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Galerie der Validierungsdaten

• 

  WB analysis using 66223-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66223-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 66223-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Jurkat using 66223-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI1 antibody (66223-1-Ig, Clone: 3C12F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, 4T1-Zellen, A375-Zellen, Daudi-Zellen, Hausschwein-Milzgewebe, Hausschwein-Thymusgewebe, Jurkat-Zellen, K-562-Zellen, Raji-Zellen, Ramos-Zellen, Rattenmilzgewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:30000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 17 publications below
• IF: See 5 publications below

Produktinformation

66223-1-Ig bindet in WB, IF, ELISA ORAI1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte, Rind
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ORAI1 fusion protein Ag18050
• Vollständiger Name: ORAI calcium release-activated calcium modulator 1
• Berechnetes Molekulargewicht: 301 aa, 33 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC015369
• Gene symbol: ORAI1
• Gene ID (NCBI): 84876
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Publikationen

Species	Application	Title
rat	WB	Cell Death Dis; Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats; Authors - Yajie Wang
human	WB,IF	Oncogenesis; Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth; Authors - Chunming Gu
human	WB	Int J Mol Sci; Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y; Authors - Ilaria Nigro
mouse	WB	Front Cell Dev Biol; Transmembrane Protein Ttyh1 Maintains the Quiescence of Neural Stem Cells Through Ca2+/NFATc3 Signaling.; Authors - Yuan Cao
mouse	WB	J Innate Immun; Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery.; Authors - Yunzhi Liu
mouse	WB	Cell Calcium; Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.; Authors - Yu Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (01-19-2023)

The Ab worked well for WB. 20ug protein loaded. Probed overnight at 4 deg.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 in 5% NFDM

ORAI1 Antibody Western Blot validation (1:2000 in 5% NFDM dilution) in (Cat no:66223-1-Ig)