OS9 Rekombinanter Antikörper

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• KD/KO Validated

OS9 Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230149B4

Kat-Nr. : 82932-1-RR

Synonyme

230149B4, Amplified in osteosarcoma 9, Protein OS-9

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  WB analysis using 82932-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82932-1-RR (OS9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 82932-1-RR

  WB result of OS9 antibody (82932-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OS9 transfected MCF-7 cells.

• 

  IHC staining of mouse testis using 82932-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 82932-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-1-RR (OS9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 82932-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-1-RR, Clone:230149B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82932-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82932-1-RR against Human OS9 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HUVEC-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82932-1-RR bindet in WB, IHC, FC (Intra), ELISA OS9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OS9 fusion protein Ag0106
• Vollständiger Name: osteosarcoma amplified 9, endoplasmic reticulum associated protein
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 83-97 kDa
• GenBank-Zugangsnummer: BC000532
• Gene symbol: OS9
• Gene ID (NCBI): 10956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.