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OSBPL10 Polyklonaler Antikörper

OSBPL10 Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-15491

Synonyme

OSBP-related protein 10, OSBP9, OSBP related protein 10, ORP-10, ORP10

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated OSBPL10 Polyclonal a (CL488-15491)

    IF Staining of HepG2 using CL488-15491

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 OSBPL10 antibody (CL488-15491) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated OSBPL10 Polyclonal a (CL488-15491)

    FC experiment of HepG2 using CL488-15491

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Osbpl10 Polyclonal Antibody (CL488-15491)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHepG2-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-15491 bindet in IF/ICC, FC (Intra) OSBPL10 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen OSBPL10 fusion protein Ag7861
Vollständiger Name oxysterol binding protein-like 10
Berechnetes Molekulargewicht 84 kDa
Beobachtetes Molekulargewicht 75-80 kDa
GenBank-ZugangsnummerBC003168
Gene symbol OSBPL10
Gene ID (NCBI) 114884
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OSBPL10, also known as ORP10, is a member of the oxysterol-binding protein family. OSBPL10 localizes on microtubules and acts as a regulator of cellular lipid biosyntheses and apolipoprotein B-100. Genetic variation in OSBPL10 is associated with dyslipidmias and peripheral artery disease.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 488 OSBPL10 antibody CL488-15491Protokoll herunterladen
FC protocol for CL Plus 488 OSBPL10 antibody CL488-15491Download protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen