OSBPL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OSBPL2 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Rezensionen (1)

Kat-Nr. : 14751-1-AP

Synonyme

OSBP-related protein 2, OSBP related protein 2, ORP-2, ORP2, ORP 2

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  WB analysis using 14751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14751-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14751-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 14751-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14751-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14751-1-AP

  IP result of anti-OSBPL2 (IP:14751-1-AP, 4ug; Detection:14751-1-AP 1:500) with HeLa cells lysate 880ug.

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  IHC staining of human ovary cancer using 14751-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14751-1-AP (OSBPL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 14751-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OSBPL2 antibody (14751-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HepG2-Zellen, HuH-7-Zellen, Maushirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 5 publications below
• ChIP: See 1 publications below

Produktinformation

14751-1-AP bindet in WB, IHC, IF-P, IP, ChIP, ELISA OSBPL2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OSBPL2 fusion protein Ag6496
• Vollständiger Name: oxysterol binding protein-like 2
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 51 kDa, 56 kDa
• GenBank-Zugangsnummer: BC000296
• Gene symbol: OSBPL2
• Gene ID (NCBI): 9885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Protein Cell; ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover; Authors - Maomao Pu
human	WB,IF	Autophagy; OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin.; Authors - Young Ik Koh
mouse	IF	JCI Insight; Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.; Authors - Hairong Shi
	WB	Genet Med; Identification of OSBPL2 as a novel candidate gene for progressive nonsyndromic hearing loss by whole-exome sequencing.; Authors - Guangqian Xing
human	IF,ChIP	Mol Cell Endocrinol; Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis.; Authors - Tamara Escajadillo ; KD Validated
human	WB	Exp Cell Res; OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.; Authors - Cui Zhang ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (11-25-2024)

recognized a band at the expected molecular weight

• Applications: Western Blot