OSTF1 Polyklonaler Antikörper

OSTF1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 10671-1-AP

Synonyme

OSF, Osteoclast-stimulating factor 1, SH3P2

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  WB analysis of C2C12 using 10671-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 10671-1-AP (OSTF1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 10671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10671-1-AP (OSTF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10671-1-AP (OSTF1 antibody) at dilution of 1:100 (under 25x lens).

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  IHC staining of human lung using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10671-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 10671-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using OSTF1 antibody (10671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C2C12-Zellen, HepG2-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Plazenta-Gewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

10671-1-AP bindet in WB, IHC, IF/ICC, ELISA OSTF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OSTF1 fusion protein Ag1072
• Vollständiger Name: osteoclast stimulating factor 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC007459
• Gene symbol: OSTF1
• Gene ID (NCBI): 26578
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OSTF1 (Osteoclast-stimulating factor-1) is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption. It induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity

Publikationen

Species	Application	Title
rat,human	WB,IHC	Heliyon; OSTF1 knockdown mitigates IL-1β-induced chondrocyte injury via inhibiting the NF-κB signaling pathway; Authors - Bin Hu