OTX2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OTX2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

26

Rezensionen (1)

Kat-Nr. : 13497-1-AP

Synonyme

Homeobox protein OTX2, MCOPS5, orthodenticle homeobox 2, Orthodenticle homolog 2, OTX2

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Galerie der Validierungsdaten

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  WB analysis using 13497-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13497-1-AP (OTX2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Y79 using 13497-1-AP

  IP Result of anti-OTX2 (IP:13497-1-AP, 3ug; Detection:13497-1-AP 1:1500) with Y79 cells lysate 2400ug.

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  IP experiment of Y79 using 13497-1-AP

  IP Result of anti-OTX2 (IP:13497-1-AP, 4ug; Detection:13497-1-AP 1:2000) with Y79 cells lysate 2000ug.

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  IHC staining of human gliomas using 13497-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13497-1-AP (OTX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human gliomas using 13497-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13497-1-AP (OTX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of mouse embryo using 13497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 13497-1-AP (OTX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse embryo using 13497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 13497-1-AP (OTX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse embryo using 13497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 13497-1-AP (OTX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of hESC cells using 13497-1-AP

  FC result of anti-OTX2 (13497-1-AP, 1:100) with hESC which spontaneous differentiation toward retinal lineage (RPE). (Black: control; Blue OTX2).

• 

  FC experiment of HepG2 using 13497-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human OTX2 (13497-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, Maus-Retina-Gewebe, Ratten-Retina-Gewebe
• Erfolgreiche IP: Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausembryogewebe
• Erfolgreiche Detektion in FC: HepG2-Zellen, hESC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below
• ChIP: See 6 publications below

Produktinformation

13497-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA OTX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OTX2 fusion protein Ag4323
• Vollständiger Name: orthodenticle homeobox 2
• Berechnetes Molekulargewicht: 297 aa, 32 kDa
• Beobachtetes Molekulargewicht: 31-35 kDa
• GenBank-Zugangsnummer: BC032579
• Gene symbol: OTX2
• Gene ID (NCBI): 5015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive

Publikationen

Species	Application	Title
human	WB	Nature; Unified rhombic lip origins of group 3 and group 4 medulloblastoma; Authors - Kyle S. Smith
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse	WB	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du ; KD Validated
mouse	IF,ChIP	Science; Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.; Authors - Catherine J Peña
human	chip	Cancer Cell; Atypical Teratoid/Rhabdoid Tumors Are Comprised of Three Epigenetic Subgroups with Distinct Enhancer Landscapes.; Authors - Pascal D Johann
mouse	WB,ChIP	Cell Rep; Otx2 and Oct4 Drive Early Enhancer Activation during Embryonic Stem Cell Transition from Naive Pluripotency.; Authors - Shen-Hsi Yang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

David (Verified Customer) (02-21-2022)

This antibody works very well for immunofluorescence on human brain.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: Human brain

OTX2 Antibody Immunofluorescence validation (1 to 200 dilution) in Human brain (Cat no:13497-1-AP)

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