OXA1L Monoklonaler Antikörper

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• KD/KO Validated

OXA1L Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

4A2D9

Kat-Nr. : 66128-1-Ig

Synonyme

Hsa, OXA1 like protein, OXA1Hs, OXA1L

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Galerie der Validierungsdaten

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  WB analysis using 66128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66128-1-Ig (OXA1L antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HEK-293 using 66128-1-Ig

  WB result of OXA1L antibody (66128-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXA1L transfected HEK-293 cells.

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  WB analysis of NIH/3T3 using 66128-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66128-1-Ig (OXA1L antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 66128-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain slide using 66128-1-Ig (OXA1L Antibody) at dilution of 1:50.

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  IHC staining of human brain using 66128-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain slide using 66128-1-Ig (OXA1L Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: T-47D-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, L02-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

66128-1-Ig bindet in WB, IHC, ELISA OXA1L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OXA1L fusion protein Ag13819
• Vollständiger Name: oxidase (cytochrome c) assembly 1-like
• Berechnetes Molekulargewicht: 437 aa, 49 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC001669
• Gene symbol: OXA1L
• Gene ID (NCBI): 5018
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.

Publikationen

Species	Application	Title
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza
human	WB	Cell; Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.; Authors - Ricarda Richter-Dennerlein
human	WB	Cell Rep; EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter.; Authors - Horia Vais
mouse	WB	Cell Rep; Mitochondrial microproteins link metabolic cues to respiratory chain biogenesis; Authors - Chao Liang