Occludin Polyklonaler Antikörper

Occludin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (7)

Anwendung

WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(737)

Rezensionen (3)

Kat-Nr. : 27260-1-AP

Synonyme

OCLN

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  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HUVEC using 27260-1-AP

  IP result of anti-Occludin (IP:27260-1-AP, 4ug; Detection:27260-1-AP 1:2000) with HUVEC cells lysate 840 ug.

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  IHC staining of human kidney using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27260-1-AP (Occludin Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27260-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27260-1-AP (Occludin Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 27260-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Occludin antibody (27260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Caco-2 using 27260-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of Caco-2 using 27260-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 27260-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Occludin antibody (27260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HepG2-Zellen, Maushirngewebe, Maus-Kolongewebe, Mausnierengewebe, Mauslebergewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche IP: HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-Fro: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: Caco-2-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:10000
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 569 publications below
• IHC: See 107 publications below
• IF: See 218 publications below
• IP: See 1 publications below

Produktinformation

27260-1-AP bindet in WB, IHC, IF/ICC, IF-Fro, IP, ELISA Occludin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Hausschwein, Huhn, Hund, Kaninchen, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Occludin fusion protein Ag26173
• Vollständiger Name: occludin
• Berechnetes Molekulargewicht: 522 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa, 32 kDa, 23-25 kDa
• GenBank-Zugangsnummer: BC029886
• Gene symbol: Occludin
• Gene ID (NCBI): 4950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini, and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Publikationen

Species	Application	Title
mouse	IHC	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	IF	J Clin Invest; A20 regulates lymphocyte adhesion in murine neuroinflammation by restricting endothelial ICOSL expression in the CNS; Authors - Lisa Johann
pig	WB	Microbiome; Zearalenone disturbs the reproductive-immune axis in pigs: the role of gut microbial metabolites; Authors - Shujin Wang
	IF	Microbiome; Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1; Authors - Hao Chen
mouse	IF	Adv Sci (Weinh); Plasma-Activated Solutions Mitigates DSS-Induced Colitis via Restoring Redox Homeostasis and Reversing Microbiota Dysbiosis; Authors - Tuanhe Sun
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (06-13-2022)

Excellent antibody for immunoblotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: colon cancer cells

FH

Balawant (Verified Customer) (05-08-2022)

This antibody I have used and is working great . 1:2000 dilution is working great for the cell line (Colonic epithelialcellr of human).

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human colonic epithelial cells and mice colon tissue

FH

Kushal (Verified Customer) (03-14-2022)

• Applications: Western Blot, Immunoprecipitation, ChIP
• Primary Antibody Dilution: 1.2000
• Cell Tissue Type: Surgical human tissue, mouse tissue, Caco-2 cells