Occludin Polyklonaler Antikörper

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• KD/KO Validated

Occludin Polyklonal Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(4)

Kat-Nr. : CL594-13409

Synonyme

occludin, OCLN

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  IF Staining of T-47D using CL594-13409

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using CoraLite®594 Occludin antibody (CL594-13409) at dilution of 1:100.

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  IF Staining of MCF-7 using CL594-13409

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 Occludin antibody (CL594-13409) at dilution of 1:600.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, T-47D-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 4 publications below

Produktinformation

CL594-13409 bindet in IF/ICC Occludin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Occludin fusion protein Ag4057
• Vollständiger Name: occludin
• Berechnetes Molekulargewicht: 522 aa, 59 kDa
• GenBank-Zugangsnummer: BC029886
• Gene symbol: Occludin
• Gene ID (NCBI): 4950
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Publikationen

Species	Application	Title
mouse	IF	J Nanobiotechnology; Orally biomimetic metal-phenolic nanozyme with quadruple safeguards for intestinal homeostasis to ameliorate ulcerative colitis; Authors - Yuanyuan Zhu
	IF	Am J Pathol; Restorative Effects of Short-Chain Fatty Acids on Corneal Homeostasis Disrupted by Antibiotic-Induced Gut Dysbiosis; Authors - Sijing Liu
	IF	Mucosal Immunol; Antibiotic-induced dysbiosis of the ocular microbiome affects corneal circadian rhythmic activity in mice; Authors - Xinwei Jiao
mouse	IF	Mucosal Immunol; Stress systems exacerbate the inflammatory response after corneal abrasion in sleep-deprived mice via the IL-17 signaling pathway; Authors - Yunxia Xue