Osteopontin Polyklonaler Antikörper
Osteopontin Polyklonal Antikörper für IHC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 25715-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Mausnierengewebe, C2C12-Zellen, HEK-293-Zellen, Rattennierengewebe |
| Erfolgreiche Detektion in IHC | humanes Magenkrebsgewebe, humanes Dünndarmgewebe, humanes Nierengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 16 publications below |
| IHC | See 10 publications below |
| IF | See 1 publications below |
Produktinformation
25715-1-AP bindet in WB, IHC, IF, ELISA Osteopontin und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Osteopontin fusion protein Ag22588 |
| Vollständiger Name | secreted phosphoprotein 1 |
| Berechnetes Molekulargewicht | 314 aa, 35 kDa |
| Beobachtetes Molekulargewicht | 66 kDa |
| GenBank-Zugangsnummer | BC007016 |
| Gene symbol | SPP1 |
| Gene ID (NCBI) | 6696 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands correspond to peptide fragments (PMID: 8195113; 17890765).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for Osteopontin antibody 25715-1-AP | Protokoll herunterladen |
| IHC protocol for Osteopontin antibody 25715-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Neurosci Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2. | ||
Sci Adv Diversified transcriptional responses of myeloid and glial cells in spinal cord injury shaped by HDAC3 activity. | ||
Part Fibre Toxicol Aryl hydrocarbon receptor activation-mediated vascular toxicity of ambient fine particulate matter: contribution of polycyclic aromatic hydrocarbons and osteopontin as a biomarker. | ||
Cell Mol Life Sci Metastasis suppressor 1 controls osteoblast differentiation and bone homeostasis through regulating Src-Wnt/β-catenin signaling. | ||
Clin Transl Immunology Deficient DNASE1L3 facilitates neutrophil extracellular traps-induced invasion via cyclic GMP-AMP synthase and the non-canonical NF-κB pathway in diabetic hepatocellular carcinoma. | ||
Stem Cells High-mobility group AT-Hook 1 mediates the role of nuclear factor I/X in osteogenic differentiation through activating canonical Wnt signaling. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Eli (Verified Customer) (01-31-2020) | Loaded 20 (lanes 2 and 3) , 10 (lanes 4 and 5), 5 (lanes 6 and 7), and 2.5 (lanes 8 and 9) ug of cell lysates to the lanes listed.
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