NOLC1 Polyklonaler Antikörper

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• KD/KO Validated

NOLC1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 11815-1-AP

Synonyme

KIAA0035, NOLC1, NOPP130, NOPP140, NS5ATP13, Nucleolar 130 kDa protein, Nucleolar phosphoprotein p130

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11815-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11815-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11815-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11815-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11815-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11815-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11815-1-AP

  IP Result of anti-NOLC1 (IP:11815-1-AP, 4ug; Detection:11815-1-AP 1:500) with HeLa cells lysate 2000ug.

• 

  IHC staining of human colon cancer using 11815-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11815-1-AP (NOLC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11815-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11815-1-AP (NOLC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11815-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11815-1-AP (NOLC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11815-1-AP

  Immunofluorescent analysis of HepG2 cells, using NOLC1 antibody 11815-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

11815-1-AP bindet in WB, IP, IHC, IF, ELISA NOLC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOLC1 fusion protein Ag2382
• Vollständiger Name: nucleolar and coiled-body phosphoprotein 1
• Berechnetes Molekulargewicht: 130 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC006769
• Gene symbol: NOLC1
• Gene ID (NCBI): 9221
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleolar and coiled-body phosphoprotein 1 (NOLC1) is a phosphoprotein that transiently associates with the mature nucleolar H/ACA and C/D box small nucleolar ribonucleoproteins (snoRNPs), guiding site-specific 2'-O-meth-ylation and pseudouridylation of pre-rRNAs [PMID:21266110]. It contains a nuclear localization signal binding sequence and is thought to shuttle between the nucleolus and the cytoplasm. NOLC1 plays an essential role in the synthesis of rRNA and the biosynthesis of ribosomes [PMID:8972203].

Publikationen

Species	Application	Title
human	IF	Nat Biotechnol; Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases; Authors - Matthew T N Yarnall
human	WB	Nature; Cell-fate determination by ubiquitin-dependent regulation of translation.; Authors - Achim Werner
human	WB,IF	Elife; Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.; Authors - Achim Werner ; KD Validated
human,mouse	WB,IHC	Acta Pharmacol Sin; Atypical chemokine receptor 3 induces colorectal tumorigenesis in mice by promoting β-arrestin-NOLC1-fibrillarin-dependent rRNA biogenesis.; Authors - Juan Yang
	WB,IF	J Cell Sci; Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.; Authors - Valentina Iadevaia
human	WB	Sci Adv; Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells; Authors - Chandreyee Datta