P2RX4 Monoklonaler Antikörper

P2RX4 Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1A5A6

Kat-Nr. : 66416-1-Ig

Synonyme

ATP receptor, P2RX4, P2X purinoceptor 4, P2X4, P2X4R, Purinergic receptor

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Galerie der Validierungsdaten

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  WB analysis using 66416-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66416-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66416-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66416-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66416-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human kidney using 66416-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66416-1-Ig (P2RX4 Antibody) at dilution of 1:400 (under 40x lens).

• 

  FC experiment of HeLa using 66416-1-Ig

  1X10^6 HeLa cells were stained with 0.20 ug Anti-Human P2RX4 (66416-1-Ig, Clone:1A5A6) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Hausschwein-Hirngewebe, humanes Hirngewebe, Kaninchenhirngewebe, Maushirngewebe, Neuro-2a-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66416-1-Ig bindet in WB, IHC, FC, ELISA P2RX4 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: P2RX4 fusion protein Ag4445
• Vollständiger Name: purinergic receptor P2X, ligand-gated ion channel, 4
• Berechnetes Molekulargewicht: 388 aa, 43 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC033826
• Gene symbol: P2RX4
• Gene ID (NCBI): 5025
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439).

Publikationen

Species	Application	Title
mouse	WB	Clin Transl Med; P2X4 receptor modulates gut inflammation and favours microbial homeostasis in colitis; Authors - Peijie Zhong

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