P2RX5 Polyklonaler Antikörper

P2RX5 Polyklonal Antikörper für FC, IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, FC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 19012-1-AP

Synonyme

ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor

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Galerie der Validierungsdaten

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  WB analysis of BxPC-3 using 19012-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 19012-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 19012-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of BxPC-3 using 19012-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed BxPC-3 cells using 19012-1-AP (P2RX5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 19012-1-AP

  1X10^6 K-562 cells were stained with 0.2ug P2RX5 antibody (19012-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HL-60-Zellen, MDA-MB-453s-Zellen
• Erfolgreiche Detektion in IF: BxPC-3-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

19012-1-AP bindet in WB, IF, FC,ELISA P2RX5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P2RX5 fusion protein Ag5297
• Vollständiger Name: purinergic receptor P2X, ligand-gated ion channel, 5
• Berechnetes Molekulargewicht: 422 aa, 47 kDa
• Beobachtetes Molekulargewicht: 44 kDa, 49 kDa, 116 kDa
• GenBank-Zugangsnummer: BC039015
• Gene symbol: P2RX5
• Gene ID (NCBI): 5026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	FC	PLoS One; A Truncation Variant of the Cation Channel P2RX5 Is Upregulated during T Cell Activation.; Authors - Pierre Abramowski

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