PABPC1,PABP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PABPC1,PABP Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (3)

Kat-Nr. : 10970-1-AP

Synonyme

PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC1,PABP, PABPC2, PABPL1, Poly(A) binding protein 1

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Galerie der Validierungsdaten

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  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10970-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10970-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10970-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10970-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10970-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10970-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 10970-1-AP

  IP Result of anti-PABPC1,PABP (IP:10970-1-AP, 4ug; Detection:10970-1-AP 1:700) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human pancreas using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 10970-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PABPC1 antibody 10970-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of MCF-7 using 10970-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug PABPC1,PABP antibody (10970-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, A549-Zellen, HeLa-Zellen, HT-29-Zellen, Maushodengewebe, MCF-7-Zellen, NCI-H1299-Zellen, PC-3-Zellen, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

10970-1-AP bindet in WB, RIP, IP, IHC, IF, FC, ELISA PABPC1,PABP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PABPC1,PABP fusion protein Ag1422
• Vollständiger Name: poly(A) binding protein, cytoplasmic 1
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC015958
• Gene symbol: PABPC1
• Gene ID (NCBI): 26986
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].

Publikationen

Species	Application	Title
human	WB	Exp Mol Med; The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability; Authors - Qianqian Yang
human	WB	Nat Commun; An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis.; Authors - Song Zhu
human	WB	J Exp Med; E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non-small cell lung cancer.; Authors - Ke Li
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
rat	IHC	Leukemia; An atlas of bloodstream-accessible bone marrow proteins for site-directed therapy of acute myeloid leukemia.; Authors - L Angenendt
mouse	WB,IP	Haematologica; YTHDF3 modulates hematopoietic stem cells by recognizing RNA m6A modification on Ccnd1.; Authors - Xiaofei Zhang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (01-13-2023)

PABPC1 (Poly(A) Binding Protein Cytoplasmic 1) staining (in magenta) and Hoechst (nuclear staining) in blue. PABPC1 ab shows a clear citoplasma staining. Method: RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 10'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

PABPC1,PABP Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:10970-1-AP)

FH

Zee (Verified Customer) (01-28-2020)

It worked very well when I performed western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

FH

George (Verified Customer) (09-11-2019)

Rb PABP Ig shows very little endogenous background staining with clear dispersed staining seen both in the nucleus and cytoplasm. 90mins heat shock at 42oC led to granular formation in the cytoplasm

• Applications: Immunohistochemistry, Other,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Spinal Cord/Cortical Slice Culture