PACT Polyklonaler Antikörper

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• KD/KO Validated

PACT Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28636-1-AP

Synonyme

DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX

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Galerie der Validierungsdaten

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  WB analysis using 28636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28636-1-AP (PACT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28636-1-AP

  WB result of PACT antibody (28636-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected HeLa cells.

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  WB analysis of MCF-7 using 28636-1-AP

  WB result of PACT antibody (28636-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected MCF-7 cells.

• 

  WB analysis using 28636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28636-1-AP (PRKRA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, Maushodengewebe, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28636-1-AP bindet in WB, ELISA PACT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PACT fusion protein Ag29784
• Vollständiger Name: protein kinase, interferon-inducible double stranded RNA dependent activator
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC009470
• Gene symbol: PRKRA
• Gene ID (NCBI): 8575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).