PAF1 Polyklonaler Antikörper

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• KD/KO Validated

PAF1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15441-1-AP

Synonyme

F23149_1, hPAF1, PAF1, PD2

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15441-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15441-1-AP (PAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15441-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15441-1-AP (PAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15441-1-AP

  IP Result of anti-PAF1 (IP:15441-1-AP, 3ug; Detection:15441-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of human ovary tumor using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15441-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15441-1-AP (PAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below

Produktinformation

15441-1-AP bindet in WB, IP, IHC, ELISA PAF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAF1 fusion protein Ag7212
• Vollständiger Name: Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC000017
• Gene symbol: PAF1
• Gene ID (NCBI): 54623
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAF1, also known as hPAF1 or PD2, is a PAF (RNA Polymerase II Associated Factor) complex subunit. Paf1 functions primarily to modulates transcript elongation. PAF1 occupies transcriptional enhancers and restrains hyperactivation of a subset of these enhancers(PMID: 28860207). The PAF complex also has a role in histone monoubiquitination. Paf1 is overexpressed in pancreatic cancer cells and has demonstrated potential oncogenic property. The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.

Publikationen

Species	Application	Title
human	WB	Autophagy; The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.; Authors - Liangde Zheng ; KD Validated
human	WB	J Cell Biol; Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.; Authors - Chen Chen
human	WB	Radiat Oncol; Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription.; Authors - Jing-Jie Zheng
human	WB	ACS Pharmacol Transl Sci; Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening; Authors - Lei Wang
human	WB	Sci Adv; CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation; Authors - Min Qiu
human	WB	Cell; SARS-CoV-2 variants evolve convergent strategies to remodel the host response; Authors - Mehdi Bouhaddou