PAICS Polyklonaler Antikörper

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• KD/KO Validated

PAICS Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12967-1-AP

Synonyme

ADE2, ADE2H1, AIR carboxylase, AIRC, PAICS, PAIS

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Galerie der Validierungsdaten

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  WB analysis using 12967-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12967-1-AP (PAICS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12967-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12967-1-AP (PAICS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 12967-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12967-1-AP (PAICS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12967-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12967-1-AP (PAICS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 12967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12967-1-AP (PAICS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12967-1-AP (PAICS antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HeLa using 12967-1-AP

  1X10^6 HeLa cells were stained with 0.2ug PAICS antibody (12967-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Mausherzgewebe, Maushirngewebe, Raji-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12967-1-AP bindet in WB, IHC, IF, FC, CoIP, ELISA PAICS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAICS fusion protein Ag3621
• Vollständiger Name: phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
• Berechnetes Molekulargewicht: 425 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC010273
• Gene symbol: PAICS
• Gene ID (NCBI): 10606
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAICS is also named as ADE2(Multifunctional protein), AIRC, PAIS. It is an important bifunctional enzyme in de novo purine biosynthesis in vertebrate with both 5-aminoimidazole ribonucleotide carboxylase (AIRc) and 4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide synthetase (SAICARs) activities. It becomes an attractive target for rational anticancer drug design, since rapidly dividing cancer cells rely heavily on the purine de novo pathway for synthesis of adenine and guanine, whereas normal cells favor the salvage pathway(PMID:17224163).

Publikationen

Species	Application	Title
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
mouse	IF,CoIP	iScience; Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex.; Authors - Seiya Yamada ; KD Validated
human	WB	Biol Res; Knockdown of PAICS inhibits malignant proliferation of human breast cancer cell lines.; Authors - Minjun Meng ; KD Validated
human	WB	J Clin Invest; Therapeutic targeting of metabolic vulnerabilities in cancers with MLL3/4-COMPASS epigenetic regulator mutations; Authors - Zibo Zhao
mouse	WB	eNeuro; Spatiotemporal Regulation of De Novo and Salvage Purine Synthesis during Brain Development; Authors - Tomoya Mizukoshi