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PAIP1 Monoklonaler Antikörper

PAIP1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Kaninchen, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H9D4

Kat-Nr. : CL488-68042

Synonyme

PABP interacting protein 1, PAIP 1, PAIP1

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PAIP1 Monoclonal ant (CL488-68042)

    IF Staining of HeLa using CL488-68042

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PAIP1 antibody (CL488-68042, Clone: 2H9D4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated PAIP1 Monoclonal ant (CL488-68042)

    FC experiment of HeLa using CL488-68042

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PAIP1 (CL488-68042, Clone:2H9D4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68042 bindet in IF, FC (Intra) PAIP1 und zeigt Reaktivität mit human, Kaninchen, Maus

Getestete Reaktivität human, Kaninchen, Maus
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen PAIP1 fusion protein Ag16773
Vollständiger Name poly(A) binding protein interacting protein 1
Berechnetes Molekulargewicht 70 kDa
Beobachtetes Molekulargewicht40-45kDa, 62kDa
GenBank-ZugangsnummerBC005295
Gene symbol PAIP1
Gene ID (NCBI) 10605
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 PAIP1 antibody CL488-68042Protokoll herunterladen
FC protocol for CL Plus 488 PAIP1 antibody CL488-68042Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen