PAIP1 Monoklonaler Antikörper

PAIP1 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Kaninchen, Maus

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H9D4

Kat-Nr. : CL488-68042

Synonyme

Poly(A)-binding protein-interacting protein 1, PAIP-1, PAIP 1, PABP-interacting protein 1, PABP interacting protein 1

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  IF Staining of HeLa using CL488-68042

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PAIP1 antibody (CL488-68042, Clone: 2H9D4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-68042

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PAIP1 (CL488-68042, Clone:2H9D4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68042 bindet in IF/ICC, FC (Intra) PAIP1 und zeigt Reaktivität mit human, Kaninchen, Maus

• Getestete Reaktivität: human, Kaninchen, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PAIP1 fusion protein Ag16773
• Vollständiger Name: poly(A) binding protein interacting protein 1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 40-45kDa, 62kDa
• GenBank-Zugangsnummer: BC005295
• Gene symbol: PAIP1
• Gene ID (NCBI): 10605
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.