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PARK2/Parkin Polyklonaler Antikörper
PARK2/Parkin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
Hausschwein, human, Maus, Ratte und mehr (4)
Anwendung
WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14060-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Maushirngewebe, C6-Zellen, HEK-293-Zellen, Mauslebergewebe, PC-12-Zellen, Hausschwein-Hirngewebe, Rattenhirngewebe |
Erfolgreiche Detektion in IHC | Mausnierengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | Mausherzgewebe, Maushirngewebe |
Erfolgreiche Detektion in IF/ICC | RAW 264.7-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 12 publications below |
WB | See 345 publications below |
IHC | See 40 publications below |
IF | See 83 publications below |
IP | See 5 publications below |
CoIP | See 6 publications below |
Produktinformation
14060-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA PARK2/Parkin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Affe, Hausschwein, Huhn, Kaninchen, Maus, Ratte, Rind |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | PARK2/Parkin fusion protein Ag5092 |
Vollständiger Name | Parkinson disease (autosomal recessive, juvenile) 2, parkin |
Berechnetes Molekulargewicht | 52 kDa |
Beobachtetes Molekulargewicht | 42-52 kDa |
GenBank-Zugangsnummer | BC022014 |
Gene symbol | Parkin |
Gene ID (NCBI) | 5071 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a variety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing with molecular weights of 24, 31, 36 and 42-52 kDa. Sometimes an additional band of 70 kDa or 110 kDa may be detected, which is caused by ubiquitination modification or formation of Parkin complex (PMID: 10976934, PMID: 18190519).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for PARK2/Parkin antibody 14060-1-AP | Protokoll herunterladen |
IHC protocol for PARK2/Parkin antibody 14060-1-AP | Protokoll herunterladenl |
IF protocol for PARK2/Parkin antibody 14060-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Cell Biol Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells | ||
Nat Commun Augmented temperature fluctuation aggravates muscular atrophy through the gut microbiota | ||
Acta Pharm Sin B Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy. | ||
Acta Pharm Sin B Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy | ||
Autophagy Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Javier (Verified Customer) (09-09-2025) | Perfect antibody for western blotting assay.
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FH QX (Verified Customer) (05-08-2025) | It is okay to detect endogenous PARK2 with the right around 52 kd MW although there are some nonspecific bands,
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FH Ajte (Verified Customer) (03-08-2022) | It works well for me
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FH Elyssa (Verified Customer) (11-11-2019) | This antibody worked well for me for one cell line (I had tried detecting the protein in 3 cells lines, and only one cell line appeared to have expression. However, I have not looked into this further and cannot say if this has to do with the antibody, or differential expression across lines). Overall, I was happy with the antibody.
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