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PARK2/Parkin Polyklonaler Antikörper

PARK2/Parkin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (4)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(384)

Kat-Nr. : 14060-1-AP

Synonyme

PARK2, Parkin, E3 ubiquitin-protein ligase parkin, EC:2.3.2.31, Parkin RBR E3 ubiquitin-protein ligase



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, C6-Zellen, HEK-293-Zellen, Mauslebergewebe, PC-12-Zellen, Hausschwein-Hirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IHCMausnierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMausherzgewebe, Maushirngewebe
Erfolgreiche Detektion in IF/ICCRAW 264.7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14060-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA PARK2/Parkin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Hausschwein, Huhn, Kaninchen, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PARK2/Parkin fusion protein Ag5092
Vollständiger Name Parkinson disease (autosomal recessive, juvenile) 2, parkin
Berechnetes Molekulargewicht 52 kDa
Beobachtetes Molekulargewicht 42-52 kDa
GenBank-ZugangsnummerBC022014
Gene symbol Parkin
Gene ID (NCBI) 5071
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a variety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing with molecular weights of 24, 31, 36 and 42-52 kDa. Sometimes an additional band of 70 kDa or 110 kDa may be detected, which is caused by ubiquitination modification or formation of Parkin complex (PMID: 10976934, PMID: 18190519).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PARK2/Parkin antibody 14060-1-APProtokoll herunterladen
IHC protocol for PARK2/Parkin antibody 14060-1-APProtokoll herunterladenl
IF protocol for PARK2/Parkin antibody 14060-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells

Authors - Huafeng Zhang
WB

Nat Commun

Augmented temperature fluctuation aggravates muscular atrophy through the gut microbiota

Authors - Ya Liu
humanIF

Mol Cell

The HRI branch of the integrated stress response selectively triggers mitophagy

Authors - Yogaditya Chakrabarty
humanWB

Acta Pharm Sin B

Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.

Authors - Xin Sun
mouseWB

Acta Pharm Sin B

Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy

Authors - Chang Xiao
humanWB

Autophagy

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Authors - Qichao Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Javier (Verified Customer) (09-09-2025)

Perfect antibody for western blotting assay.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mice kidneys
FH

QX (Verified Customer) (05-08-2025)

It is okay to detect endogenous PARK2 with the right around 52 kd MW although there are some nonspecific bands,

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain lysate
PARK2/Parkin Antibody Western Blot validation (1:1000 dilution) in mouse brain lysate (Cat no:14060-1-AP)
FH

Ajte (Verified Customer) (03-08-2022)

It works well for me

  • Applications: Western Blot
FH

Elyssa (Verified Customer) (11-11-2019)

This antibody worked well for me for one cell line (I had tried detecting the protein in 3 cells lines, and only one cell line appeared to have expression. However, I have not looked into this further and cannot say if this has to do with the antibody, or differential expression across lines). Overall, I was happy with the antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T47D, MCF7, CAMA1 (only worked in T47D)