PARP11 Polyklonaler Antikörper

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• KD/KO Validated

PARP11 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16692-1-AP

Synonyme

ADP-ribosyltransferase diphtheria toxin-like 11, ARTD11, C12orf6, EC:2.4.2.-, MIB006

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  WB analysis using 16692-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16692-1-AP

  WB result of PARP11 antibody (16692-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PARP11 transfected HeLa cells.

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  WB analysis of PC-3 using 16692-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 16692-1-AP (PARP11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 16692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 16692-1-AP (PARP11 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mausherzgewebe, Mausmilzgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolongewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

16692-1-AP bindet in WB, IHC, IF, ELISA PARP11 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PARP11 fusion protein Ag10053
• Vollständiger Name: poly (ADP-ribose) polymerase family, member 11
• Berechnetes Molekulargewicht: 331 aa, 39 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC017569
• Gene symbol: PARP11
• Gene ID (NCBI): 57097
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARP11, also named as ARTD11, belongs to the  ARTD/PARP family. PARP11 is a mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. PARP11 plays a role in nuclear envelope stability and nuclear remodeling during spermiogenesis (PMID: 25043379). PARP11 has 4 isoforms with the molecular mass of 28-40 kDa.

Publikationen

Species	Application	Title
pig,human	WB,IF	Front Cell Infect Microbiol; Identification of porcine PARP11 as a restricted factor for pseudorabies virus; Authors - Chunyun Qi ; KD Validated