PARP3 Polyklonaler Antikörper

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• KD/KO Validated

PARP3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 11289-1-AP

Synonyme

hPARP 3, EC:2.4.2.-, DNA ADP-ribosyltransferase PARP3, ARTD3, ADPRT3

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  WB analysis using 11289-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11289-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11289-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11289-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11289-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11289-1-AP (PARP3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 11289-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11289-1-AP

  Immunofluorescent analysis of Hela cells, using PARP3 antibody 11289-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, humanes Nierengewebe, Mausnierengewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

11289-1-AP bindet in WB, IHC, IF/ICC, ELISA PARP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PARP3 fusion protein Ag1844
• Vollständiger Name: poly (ADP-ribose) polymerase family, member 3
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60-62 kDa
• GenBank-Zugangsnummer: BC014260
• Gene symbol: PARP3
• Gene ID (NCBI): 10039
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARP3 (Poly(ADP-ribose) polymerase 3), also known as ARTD3, is the third member of the PARP family that catalyze a post-translational modification of proteins to promote, control or adjust numerous cellular events including genome integrity, transcription, differentiation, cell metabolism or cell death (PMID: 31095444). PARP3 is a 60-kDa protein containing an N-terminal WGR (tryptophan-, glycine-, and arginine-rich) domain and a C-terminal catalytic domain. PARP3 has been described to interact with partners belonging to the NHEJ pathway including DNA-PKcs, DNA ligase IV, Ku70 and Ku80 and to accelerate XRCC4/DNA ligase IV-mediated ligation of chromosomal DSB in concert with APLF (PMID: 24598253).

Publikationen

Species	Application	Title
human	WB	Mol Cell; Unrestrained poly-ADP-ribosylation provides insights into chromatin regulation and human disease.; Authors - Evgeniia Prokhorova
	WB	EBioMedicine; PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response; Authors - Wei Yue
human	WB	Cell Chem Biol; Allosteric regulation of DNA binding and target residence time drive the cytotoxicity of phthalazinone-based PARP-1 inhibitors; Authors - Moriah R Arnold ; KD Validated