Alpha Parvin/Actopaxin Polyklonaler Antikörper

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• KD/KO Validated

Alpha Parvin/Actopaxin Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 55268-1-AP

Synonyme

Alpha parvin, Actopaxin, PARVA, α-Parvin, CH-ILKBP

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  WB analysis of U2OS using 55268-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55268-1-AP

  WB result of α-Parvin/Actopaxin antibody (55268-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.

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  WB analysis of mouse kidney using 55268-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 55268-1-AP

  IP result of anti-Alpha Parvin/Actopaxin (IP:55268-1-AP, 4ug; Detection:55268-1-AP 1:1000) with U2OS cells lysate 0 ug.

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  IHC staining of human stomach cancer using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 55268-1-AP (Alpha Parvin/Actopaxin antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MDCK using 55268-1-AP

  Immunofluorescent analysis of MDCK cells using 55268-1-AP (PARVA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, HeLa-Zellen, Mausnierengewebe
• Erfolgreiche IP: U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Herzgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

55268-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Alpha Parvin/Actopaxin und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: parvin, alpha
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: NM_018222
• Gene symbol: Alpha Parvin/PARVA
• Gene ID (NCBI): 55742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

α-Parvin (PARVA), also named as MXRA2, Actopaxin and CH-ILKBP, belongs to the parvin family. It probably plays a role in the regulation of cell adhesion and cytoskeleton organization. (PMID:11134073) This antibody is specific to α-Parvin.

Publikationen

Species	Application	Title
mouse	WB,CoIP	Proteome Sci; Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.; Authors - Lancia N F Darville
human	WB	Nat Commun; Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis; Authors - Yesheng Fu