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  • Featured Product
  • KD/KO Validated

PATL1 Polyklonaler Antikörper

PATL1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21631-1-AP

Synonyme

PAT1 like protein 1, patellin1, PATL1, Protein PAT1 homolog 1

Filter:
  • Western Blot (WB) analysis of various lysates using PATL1 Polyclonal antibody (21631-1-AP)

    WB analysis using 21631-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21631-1-AP (PATL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PATL1 Polyclonal antibody (21631-1-AP)

    WB analysis of HeLa using 21631-1-AP

    WB result of PATL1 antibody (21631-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PATL1 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of K-562 cells using PATL1 Polyclonal antibody (21631-1-AP)

    IP experiment of K-562 using 21631-1-AP

    IP result of anti-PATL1 (IP:21631-1-AP, 4ug; Detection:21631-1-AP 1:300) with K-562 cells lysate 3320ug.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF-7-Zellen, HeLa-Zellen, K-562-Zellen
Erfolgreiche IPK-562-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

21631-1-AP bindet in WB, IP, ELISA PATL1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PATL1 fusion protein Ag16247
Vollständiger Name protein associated with topoisomerase II homolog 1 (yeast)
Berechnetes Molekulargewicht 770 aa, 87 kDa
Beobachtetes Molekulargewicht87 kDa
GenBank-ZugangsnummerBC109038
Gene symbol PATL1
Gene ID (NCBI) 219988
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Patellin1 (PATL1) is a human homolog to yeast P body component Pat1, localizes to P bodies and is required for P body formation. Furthermore, Pat1 is the only decapping activator protein that, besides interacting with deadenylated mRNAs, also binds to eIF4G-, eIF4E- and Pab1p-associated polyadenylated mRNA and locates to polysomes.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PATL1 antibody 21631-1-APProtokoll herunterladen
IP protocol for PATL1 antibody 21631-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Aditya (Verified Customer) (10-08-2024)

okay

  • Applications: Western Blot