PAX2 Polyklonaler Antikörper

PAX2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 29307-1-AP

Synonyme

paired box 2, Paired box protein Pax 2, PAX2

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  WB analysis using 29307-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29307-1-AP (PAX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• FC: See 1 publications below

Produktinformation

29307-1-AP bindet in WB, IHC, ELISA PAX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAX2 fusion protein Ag30717
• Vollständiger Name: paired box 2
• Berechnetes Molekulargewicht: 431 aa, 46 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC148710
• Gene symbol: PAX2
• Gene ID (NCBI): 5076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAX2 is a transcription factor, primarily expressed during embryonic development. It has a critical role in the development of the urogenital tract, the eyes, and the CNS. It plays a key role during renal development and to act as an oncogene favoring renal tumor growth. PAX2 was suggested as a sensitive and highly specific marker for renal cell carcinoma (RCC).

Publikationen

Species	Application	Title
human	IHC	Sci Bull (Beijing); Reprogramming of Ovarian Granulosa Cells by YAP1 Leads to Development of High-Grade Cancer with Mesenchymal Lineage and Serous Features.; Authors - Xiangmin Lv
human	FC	Cells; Human iPSC-Derived Renal Cells Change Their Immunogenic Properties during Maturation: Implications for Regenerative Therapies.; Authors - Bella Rossbach
human	WB	J Cell Mol Med; Chemerin's Role in Endometrial Dysfunction: Insights From Transcriptomic Analysis; Authors - Ming Yu