PAX8 Monoklonaler Antikörper

PAX8 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

CloneNo.

4H7B3

Rezensionen (2)

Kat-Nr. : 60145-4-Ig

Synonyme

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8

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  WB analysis of SKOV-3 using 60145-4-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human thyroid gland using 60145-4-Ig

  human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma tissue using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using DA00001(PAX8 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma tissue using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide usingDA00001 (PAX8 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 60145-4-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PAX8 antibody (60145-4-Ig, Clone: 4H7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SKOV-3 using 60145-4-Ig

  Confocal immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using 60145-4-Ig (PAX8 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Phalloidin stains F-actin (Red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, human thyroid gland tissue
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe, humanes Tonsillitisgewebe, Mausnierengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:5000-1:20000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Produktinformation

60145-4-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA PAX8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PAX8 fusion protein Ag0306
• Vollständiger Name: paired box 8
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC001060
• Gene symbol: PAX8
• Gene ID (NCBI): 7849
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).

Publikationen

Species	Application	Title
human	IF	Dev Cell; Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition; Authors - John-Poul Ng-Blichfeldt
human	IHC	Mod Pathol; Xp11.2 translocation renal cell carcinoma with NONO-TFE3 gene fusion: morphology, prognosis, and potential pitfall in detecting TFE3 gene rearrangement.; Authors - Qiu-Yuan Xia
human	IF	Stem Cell Res Ther; Genetic correction of induced pluripotent stem cells from a DFNA36 patient results in morphologic and functional recovery of derived hair cell-like cells; Authors - Yi Luo
mouse	IHC,IF	Front Cell Dev Biol; Lgr4 Regulates Oviductal Epithelial Secretion Through the WNT Signaling Pathway; Authors - Xue Tan
human	IHC	Sci Rep; Differential epithelial and stromal LGR5 expression in ovarian carcinogenesis.; Authors - Hyesung Kim
human	IHC	Hum Pathol; Clinicopathological and Molecular Characterization of Biphasic Hyalinizing Psammomatous Renal Cell Carcinoma (BHP RCC): Further Support for the Newly Proposed Entity.; Authors - Xiao-Tong Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (03-28-2024)

It worked well for immunolabeling in mouse retina tissues

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse retina

FH

Juan (Verified Customer) (04-18-2019)

It works well in Western Blot experiment.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2 Cell line