PCF11 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PCF11 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 23540-1-AP

Synonyme

KIAA0824, PCF11

---

Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 23540-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23540-1-AP (PCF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23540-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23540-1-AP (PCF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

23540-1-AP bindet in WB, CoIP, ELISA PCF11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCF11 fusion protein Ag20263
• Vollständiger Name: PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 1555 aa, 173 kDa
• Beobachtetes Molekulargewicht: 173 kDa
• GenBank-Zugangsnummer: BC146778
• Gene symbol: PCF11
• Gene ID (NCBI): 51585
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In Saccharomyces cerevisiae, the cleavage/polyadenylation factor Pcf11 is a crucial regulatory factor required for recruiting polyadenylation machinery to elongating RNA polymerase II (RNAPII), and is necessary for correct transcriptional termination. Pcf11 (PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)), is a 1,555 amino acid nuclear protein that is a component of pre-mRNA cleavage complex II. It is suggested that Pcf11 is capable of promoting the dissociation of Pol II elongation complexes from DNA. Pcf11 contains a CTD-interaction domain that binds in a phospho-dependent manner to the heptad repeats within the RNA polymerase II CTD. The gene encoding Pcf11 is located on human chromosoem 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11. This antibody is specific to the 173kd human PCF11 protein.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
human	CoIP	Neuron; Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.; Authors - Geneva R LaForce
human	WB	Nucleic Acids Res; CRISPRpas: programmable regulation of alternative polyadenylation by dCas9.; Authors - Jihae Shin ; KD Validated
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB	Biochimie; An investigation into the role of ATP in the mammalian pre-mRNA 3' cleavage reaction.; Authors - Asya Khleborodova