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PCNA Monoklonaler Antikörper
PCNA Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Maus, Ratte und mehr (2)
Anwendung
WB, IP, IHC, IF, FC, ELISA
Konjugation
Unkonjugiert
CloneNo.
10D10E11
Kat-Nr. : 60097-1-Ig
Synonyme
"PCNA Antibodies" Comparison
View side-by-side comparison of PCNA antibodies from other vendors to find the one that best suits your research needs.
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293-Zellen, 3T3-L1-Zellen, 4T1-Zellen, C6-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen, ROS1728-Zellen |
| Erfolgreiche IP | HepG2-Zellen |
| Erfolgreiche Detektion in IHC | humanes Gliomgewebe, humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Tonsillitisgewebe, Maus-Eierstockgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | HepG2-Zellen, Mausherzgewebe |
| Erfolgreiche Detektion in FC | MCF-7-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
| Immunfluoreszenz (IF) | IF : 1:250-1:1000 |
| Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 66 publications below |
| IHC | See 27 publications below |
| IF | See 19 publications below |
| IP | See 1 publications below |
Produktinformation
60097-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA PCNA und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Kaninchen, Maus, Ratte, Zebrafisch |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | PCNA fusion protein Ag7416 |
| Vollständiger Name | proliferating cell nuclear antigen |
| Berechnetes Molekulargewicht | 29 kDa/31 kDa |
| Beobachtetes Molekulargewicht | 36-38 kDa |
| GenBank-Zugangsnummer | BC000491 |
| Gene symbol | PCNA |
| Gene ID (NCBI) | 5111 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for PCNA antibody 60097-1-Ig | Protokoll herunterladen |
| IHC protocol for PCNA antibody 60097-1-Ig | Protokoll herunterladen |
| IF protocol for PCNA antibody 60097-1-Ig | Protokoll herunterladen |
| IP protocol for PCNA antibody 60097-1-Ig | Protokoll herunterladen |
| FC protocol for PCNA antibody 60097-1-Ig | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Commun USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. | ||
Nat Commun Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing. | ||
Microbiome Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets. | ||
Environ Pollut Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling | ||
Biofabrication Scale-out production of extracellular vesicles derived from natural killer cells via mechanical stimulation in a seesaw-motion bioreactor for cancer therapy. | ||
JCI Insight RNF112-mediated FOXM1 ubiquitination suppresses the proliferation and invasion of gastric cancer |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Andrea (Verified Customer) (01-11-2023) | primary antibody were incubated at 4C, ON. Antibody provide a specific signal for mouse Pcna protein
 |
FH Hasan (Verified Customer) (11-04-2022) | U2OS cells fixed with Methanol:Aceton (7:3), 20 min, 4 degrees. Blocked for 1h with 5% FBS in Triton-x 0.1% Stained with the anti-PCNA (60097-1-Ig) (1:200) O/N @4 degress
 |
FH Maria (Verified Customer) (08-07-2021) | PCNA in human primary fibroblasts in culture. Passage number 15-25. 15 ug of protein. 4-15% TGX gel. Blocking BSA 3% in PBST. Primary ab 1:1000 in BSA O/N 4ºC. Secondary ab 1:5000 HRP.
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