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PCNA Polyclonal antibody
PCNA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10205-2-AP
"PCNA Antibodies" Comparison
View side-by-side comparison of PCNA antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in||4T1 cells, A431 cells, NIH/3T3 cells, HepG2 cells, mouse testis tissue, HeLa cells, Raji cells, Jurkat cells, mouse liver tissue, HEK-293 cells, rat liver tissue, rat testis tissue, HEK293 cells, MCF-7 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human stomach cancer tissue, human malignant melanoma tissue, mouse liver tissue, human liver cancer tissue, human colon cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, Neuro-2a cells, MCF-7 cells, human breast cancer tissue|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10205-2-AP targets PCNA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, Alligator, bovine, chicken, ducks, fish, medaka embryos, pig, rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PCNA fusion protein Ag0277|
|Full Name||proliferating cell nuclear antigen|
|Calculated molecular weight||29 kDa/31 kDa|
|Observed molecular weight||36-38 kDa|
|GenBank accession number||BC000491|
|Gene ID (NCBI)||5111|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
|Product Specific Protocols|
|WB protocol for PCNA antibody 10205-2-AP||Download protocol|
|IHC protocol for PCNA antibody 10205-2-AP||Download protocol|
|IF protocol for PCNA antibody 10205-2-AP||Download protocol|
|IP protocol for PCNA antibody 10205-2-AP||Download protocol|
|FC protocol for PCNA antibody 10205-2-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Physiol
Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome.
Involvement of stromal cell-derived factor-1α (SDF-1α), stem cell factor (SCF), fractalkine (FKN) and VEGF in TSG protection against intimal hyperplasia in rat balloon injury.
Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4.
Biomed Res Int
Chlorogenic Acid Functions as a Novel Agonist of PPARγ2 during the Differentiation of Mouse 3T3-L1 Preadipocytes.
Int J Food Sci Nutr
Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.
Exp Ther Med
Rhynchophylline attenuates allergic bronchial asthma by inhibiting transforming growth factor-β1-mediated Smad and mitogen-activated protein kinase signaling transductions in vivo and in vitro.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (11-29-2021)
Nice antibody which worked well on PC3 cells fixed with 4% PFA. Antibody used at 1:50