PCNA Polyclonal antibody

• Featured Product
• KD/KO Validated

PCNA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (8)

Applications

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Conjugate

Unconjugated

Publications

702

Reviews (1)

Cat no : 10205-2-AP

Synonyms

PCNA

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis of HEK293 cells using 10205-2-AP

  WB result of PCNA antibody (10205-2-AP, 1:10000) with si-control and si-PCNA transfected HEK293 cells.

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  WB analysis using 10205-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10205-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10205-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10205-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10205-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10205-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10205-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10205-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10205-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10205-2-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 10205-2-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10205-2-AP

  IP Result of anti-PCNA (IP:10205-2-AP, 3ug; Detection:10205-2-AP 1:1500) with MCF-7 cells lysate 2500ug.

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  IP experiment using 10205-2-AP

  IP result of anti-PCNA (10205-2-AP for IP and Detection) with MCF7 cell.

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  IHC staining of human stomach cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10205-2-AP (PCNA antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human malignant melanoma using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 40x lens)..

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  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10205-2-AP (PCNA antibody) at dilution of 1:500 (under 10x lens).

• 

  IF Staining of HepG2 using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCNA antibody (10205-2-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL94-phalloidin (red), DAPI (Blue).

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  IF Staining of HepG2 using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10205-2-AP (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PCNA antibody (10205-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCNA antibody (10205-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Neuro-2a using 10205-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using 10205-2-AP (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 10205-2-AP (PCNA antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 10205-2-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human PCNA (10205-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: A431 cells, NIH/3T3 cells, mouse testis tissue, Raji cells, Jurkat cells, mouse liver tissue, rat liver tissue, rat testis tissue, HEK293 cells, HepG2 cells, HeLa cells, MCF-7 cells, PC-12 cells, C2C12 cells, mouse spleen tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human liver cancer tissue, human stomach cancer tissue, human malignant melanoma tissue, human breast cancer tissue, human colon cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, human breast cancer tissue, MCF-7 cells, mouse testis tissue, Neuro-2a cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:300-1:1200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 525 publications below
• IHC: See 181 publications below
• IF: See 58 publications below
• CoIP: See 1 publications below

Product Information

10205-2-AP targets PCNA in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, Alligator, bovine, chicken, ducks, fish, hamster, medaka embryos, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCNA fusion protein Ag0277
• Full Name: proliferating cell nuclear antigen
• Calculated molecular weight: 29 kDa/31 kDa
• Observed molecular weight: 36-38 kDa
• GenBank accession number: BC000491
• Gene symbol: PCNA
• Gene ID (NCBI): 5111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

Publications

Species	Application	Title
human	WB	Biomaterials; PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy; Authors - Lu Wang
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun
human	WB, IHC	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
human	WB	Nat Commun; Alpha-enolase regulates the malignant phenotype of pulmonary artery smooth muscle cells via the AMPK-Akt pathway.; Authors - Jingbo Dai
human	IF	Dev Cell; Cdk1 Activates Pre-mitotic Nuclear Envelope Dynein Recruitment and Apical Nuclear Migration in Neural Stem Cells.; Authors - Alexandre D Baffet
human	IHC	Nat Commun; CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.; Authors - Yuan Fang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Nice antibody which worked well on PC3 cells fixed with 4% PFA. Antibody used at 1:50

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: PC3 cells

PCNA Antibody Immunofluorescence validation (1:50 dilution) in PC3 cells (Cat no:10205-2-AP)

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