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PCNA Polyclonal antibody

PCNA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (8)

Applications

WB, IP, IHC, IF, FC

Conjugate

Unconjugated

Cat no : 10205-2-AP

Synonyms

PCNA


"PCNA Antibodies" Comparison

View side-by-side comparison of PCNA antibodies from other vendors to find the one that best suits your research needs.
  • WB analysis using 10205-2-AP

    WB analysis using 10205-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 10205-2-AP

    IF Staining of HepG2 using 10205-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCNA antibody (10205-2-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL94-phalloidin (red), DAPI (Blue).

  • FC experiment of Jurkat using 10205-2-AP

    FC experiment of Jurkat using 10205-2-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human PCNA (10205-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis of HEK293 cells using 10205-2-AP

    WB analysis of HEK293 cells using 10205-2-AP

    WB result of PCNA antibody (10205-2-AP, 1:10000) with si-control and si-PCNA transfected HEK293 cells.

  • IP experiment of MCF-7 using 10205-2-AP

    IP experiment of MCF-7 using 10205-2-AP

    IP Result of anti-PCNA (IP:10205-2-AP, 3ug; Detection:10205-2-AP 1:1500) with MCF-7 cells lysate 2500ug.

  • IHC staining of human stomach cancer using 10205-2-AP

    IHC staining of human stomach cancer using 10205-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10205-2-AP (PCNA antibody) at dilution of 1:500 (under 40x lens).

  • IF Staining of HepG2 using 10205-2-AP

    IF Staining of HepG2 using 10205-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10205-2-AP (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).


Tested Applications

Positive WB detected in4T1 cells, A431 cells, NIH/3T3 cells, HepG2 cells, mouse testis tissue, HeLa cells, Raji cells, Jurkat cells, mouse liver tissue, HEK-293 cells, rat liver tissue, rat testis tissue, HEK293 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman stomach cancer tissue, mouse heart tissue, human malignant melanoma tissue, human liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Neuro-2a cells, human breast cancer tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10205-2-AP targets PCNA in WB, IP, IHC, IF, FC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Alligator, bovine, chicken, ducks, fish, human, medaka embryos, mouse, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCNA fusion protein Ag0277
Full Name proliferating cell nuclear antigen
Calculated molecular weight 29 kDa/31 kDa
Observed molecular weight 36-38 kDa
GenBank accession numberBC000491
Gene symbol PCNA
Gene ID (NCBI) 5111
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

Protocols

Product Specific Protocols
WB protocol for PCNA antibody 10205-2-APDownload protocol
IHC protocol for PCNA antibody 10205-2-APDownload protocol
IF protocol for PCNA antibody 10205-2-APDownload protocol
IP protocol for PCNA antibody 10205-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome.

Authors - Yulin Li
ratIHC

Biomed Pharmacother

Involvement of stromal cell-derived factor-1α (SDF-1α), stem cell factor (SCF), fractalkine (FKN) and VEGF in TSG protection against intimal hyperplasia in rat balloon injury.

Authors - Anling Hu

Front Immunol

Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4.

Authors - Mudan Zhang
mouseWB

Biomed Res Int

Chlorogenic Acid Functions as a Novel Agonist of PPARγ2 during the Differentiation of Mouse 3T3-L1 Preadipocytes.

Authors - Shu-Guang Peng
humanWB

Int J Food Sci Nutr

Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.

Authors - Miaomiao Ge
mouseIF

Exp Ther Med

Rhynchophylline attenuates allergic bronchial asthma by inhibiting transforming growth factor-β1-mediated Smad and mitogen-activated protein kinase signaling transductions in vivo and in vitro.

Authors - Meng Wang