PCP4 Polyklonaler Antikörper

PCP4 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 14705-1-AP

Synonyme

Brain specific antigen PCP 4, Brain-specific polypeptide PEP-19, Calmodulin regulator protein PCP4, PEP 19, PEP19

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  WB analysis using 14705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14705-1-AP (PCP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14705-1-AP

  IP result of anti-PCP4 (IP:14705-1-AP, 4ug; Detection:14705-1-AP 1:1000) with mouse brain tissue lysate 4000 ug.

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  IHC staining of mouse cerebellum using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14705-1-AP (PCP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14705-1-AP (PCP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14705-1-AP (PCP4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14705-1-AP (PCP4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14705-1-AP (PCP4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14705-1-AP (PCP4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 14705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PCP4 antibody (14705-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse cerebellum using 14705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using PCP4 antibody (14705-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, rat brain
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Cerebellum-Gewebe, Maushirngewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Cerebellum-Gewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Produktinformation

14705-1-AP bindet in WB, IHC, IF-P, IP, ELISA PCP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCP4 fusion protein Ag6388
• Vollständiger Name: Purkinje cell protein 4
• Berechnetes Molekulargewicht: 7 kDa
• Beobachtetes Molekulargewicht: 8 kDa
• GenBank-Zugangsnummer: BC013791
• Gene symbol: PCP4
• Gene ID (NCBI): 5121
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCP4, also named as PEP19, belongs to a family of proteins involved in calcium transduction signals and binds calmodulin via an IQ motif, in a calcium independent manner. It is mainly expressed in ectoderm and neuroectoderm comprising neural crest derived cells.

Publikationen

Species	Application	Title
human	IF	Cancer Cell; Single-Cell Characterization of Malignant Phenotypes and Developmental Trajectories of Adrenal Neuroblastoma.; Authors - Rui Dong
human	IF	Cell Stem Cell; Human brain organoids assemble functionally integrated bilateral optic vesicles.; Authors - Elke Gabriel
human	IF	Nat Commun; Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening; Authors - Anand Ramani
human	WB	Ann Neurol; PMID: 32167609; Authors - Jimena Monzón-Sandoval
	IHC	Hum Genet; Human organoids for rapid validation of gene variants linked to cochlear malformations; Authors - Mohammad Faraz Zafeer
human	WB	J Pediatr Surg; CAMK2G Promotes Neuronal Differentiation and Inhibits Migration in Neuroblastoma; Authors - Yujie Ma