PD-1/CD279 Monoklonaler Antikörper

PD-1/CD279 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H4D1

Kat-Nr. : CL488-66220

Synonyme

CD279, hPD 1, hPD l, PD 1, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

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Galerie der Validierungsdaten

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  IF Staining of human tonsillitis using CL488-66220

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66220 (PD-1/CD279 antibody), at dilution of 1:150.

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  FC experiment of Jurkat using CL488-66220

  1X10^6 Jurkat cells untreated (blue, dashed line) or treated with CD3 antibody (OKT3, 2 ug/ml) were surface stained with 0.4 ug CoraLite® Plus 488 Anti-Human PD-1/CD279 (CL488-66220, Clone: 4H4D1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue, solid line). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: CD3 antibody treated Jurkat cells

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66220 bindet in IF, FC PD-1/CD279 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PD-1/CD279 fusion protein Ag12470
• Vollständiger Name: programmed cell death 1
• Berechnetes Molekulargewicht: 288 aa, 32 kDa
• GenBank-Zugangsnummer: BC074740
• Gene symbol: PDCD1
• Gene ID (NCBI): 5133
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438). This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.