CCM3/PDCD10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CCM3/PDCD10 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 10294-2-AP

Synonyme

CCM3, CCM3/PDCD10, PDCD10, programmed cell death 10, TFAR15

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Galerie der Validierungsdaten

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  WB analysis using 10294-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10294-2-AP

  IP result of anti-CCM3/PDCD10(IP:10294-2-AP, 4ug; Detection:10294-2-AP 1:500) with MCF-7 cells lysate 2120 ug.

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  IHC staining of human colon cancer using 10294-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10294-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, PC-3-Zellen, Raji-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

10294-2-AP bindet in WB, IP, IHC, IF, ELISA CCM3/PDCD10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCM3/PDCD10 fusion protein Ag0348
• Vollständiger Name: programmed cell death 10
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC002506
• Gene symbol: PDCD10
• Gene ID (NCBI): 11235
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development. Catalog#10294-2-AP is a rabbit polyclonal antibody raised against the full-length PDCD10 of human origin.

Publikationen

Species	Application	Title
mouse	WB	Nat Cell Biol; CCM3 is a gatekeeper in focal adhesions regulating mechanotransduction and YAP/TAZ signalling.; Authors - Shan Wang ; KO Validated
human,mouse	WB,IHC	J Clin Invest; Mutations in 2 distinct genetic pathways result in cerebral cavernous malformations in mice.; Authors - Chan Aubrey C AC ; KD Validated
mouse	WB	Development; Ccm3, a gene associated with cerebral cavernous malformations, is required for neuronal migration.; Authors - Angeliki Louvi ; KO Validated
mouse	WB	Aging (Albany NY); PDCD10 promotes the aggressive behaviors of pituitary adenomas by up-regulating CXCR2 and activating downstream AKT/ERK signaling; Authors - Jingdian Liu ; KO Validated
human,rat	WB,IF	J Cell Sci; CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation.; Authors - Fidalgo Miguel M ; KD Validated
human	WB	Hum Mutat; A single-center study on 140 patients with cerebral cavernous malformations: 28 new pathogenic variants and functional characterization of a PDCD10 large deletion ​.; Authors - Grazia Nardella

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